Single Nucleotide Polymorphisms at +191 and +292 of Galectin-3 Gene (LGALS3) Related to Lower GAL-3 Serum Levels Are Associated with Frequent Respiratory Tract Infection and Vaso-Occlusive Crisis in Children with Sickle Cell Anemia

• Published in: PloS one 2016-09, Vol.11 (9), p.e0162297-e0162297
• Main Authors: Mendonça Belmont, Taciana Furtado de, do Ó, Kleyton Palmeira, Soares da Silva, Andreia, de Melo Vilar, Kamila, Silva Medeiros, Fernanda, Silva Vasconcelos, Luydson Richardson, Mendonça Dos Anjos, Ana Claudia, Domingues Hatzlhofer, Betânia Lucena, Pitta, Maíra Galdino da Rocha, Bezerra, Marcos André Cavalcanti, Araújo, Aderson da Silva, de Melo Rego, Moacyr Jesus Barreto, Moura, Patrícia, Cavalcanti, Maria do Socorro Mendonça
• Format: Article
• Language: English
• Subjects: Anemia; Anemia, Sickle Cell - blood; Anemia, Sickle Cell - genetics; Apoptosis; Biology and Life Sciences; Blood; Blood Vessels - pathology; Brazil; Child; Child, Preschool; Children; Chronic infection; Clinical outcomes; Damage patterns; Diseases; Enzyme-linked immunosorbent assay; Female; Galectin 3 - blood; Galectin 3 - genetics; Galectin-3; Genes; Genetic aspects; Genotype; Genotypes; Health aspects; Hemolytic anemia; Humans; Infections; Inflammation; Male; Medicine and Health Sciences; Neutrophils; Occlusion; Oxidative stress; Oxygen; Patients; Pattern recognition; Pattern recognition receptors; Pneumonia; Pollution control; Polymorphism, Single Nucleotide - genetics; Reactive oxygen species; Respiratory tract; Respiratory tract diseases; Respiratory tract infection; Respiratory Tract Infections - blood; Respiratory Tract Infections - genetics; Serum levels; Sickle cell anemia; Sickle cell disease; Single nucleotide polymorphisms; Single-nucleotide polymorphism; VOCs; Volatile organic compounds
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0162297

Patients with sickle cell anemia (SCA) may present chronic hemolytic anemia, vaso-occlusion and respiratory tract infection (RTI) episodes. Galectin-3 (GAL-3) is a multifunctional protein involved in inflammation, apoptosis, adhesion and resistance to reactive oxygen species. Studies point to a dual role for GAL-3 as both a circulation damage-associated molecular pattern and a cell membrane associated pattern recognition receptor. To investigate associations between the SNPs of GAL-3 gene (LGALS3) and serum levels with RTI and vaso-occlusive crisis (VOC) in children with SCA. SNPs +191 and +292 in LGALS3 were studied using the TaqMan real-time PCR system; GAL-3 serum levels were measured by ELISA. The study included 79 children with SCA ranging from 2 to 12 years old. GAL-3 serum levels were associated with LGALS3 +191 and +292 genotypes (p