Genome wide identification of wheat and Brachypodium type one protein phosphatases and functional characterization of durum wheat TdPP1a

• Published in: PloS one 2018-01, Vol.13 (1), p.e0191272-e0191272
• Main Authors: Bradai, Mariem, Mahjoubi, Habib, Chini, Andrea, Chabouté, Marie-Edith, Hanin, Moez, Ebel, Chantal
• Format: Article
• Language: English
• Subjects: Abiotic stress; Analysis; Arabidopsis; Baking yeast; Biochemistry, Molecular Biology; Biology and Life Sciences; Brachypodium; Brachypodium distachyon; Catalysis; Catalytic activity; Cell cycle; Cellular signal transduction; Cellular stress response; Conserved sequence; Dephosphorylation; Ecology and Environmental Sciences; Environmental stress; Genes; Genome-wide association studies; Genomes; Heat stress; Heat tolerance; Identification; Isoforms; Kinases; Leaves; Life Sciences; Localization; Mammals; Metabolism; Metallography; Molecular biology; Nuclei; Nuclei (cytology); Oryza; Oryza sativa; Phosphatase; Phosphatases; Phosphorylation; Phylogenetics; Physiological aspects; Polyethylene glycol; Protein binding; Protein phosphatase; Proteins; Regulation; Regulatory proteins; Research and Analysis Methods; Saccharomyces cerevisiae; Salinity; Salts; Seedlings; Substrate specificity; Substrates; Tobacco; Transcription; Transduction; Triticum; Triticum aestivum; Triticum durum; Wheat; Yeast
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0191272

Reversible phosphorylation is an essential mechanism regulating signal transduction during development and environmental stress responses. An important number of dephosphorylation events in the cell are catalyzed by type one protein phosphatases (PP1), which catalytic activity is driven by the binding of regulatory proteins that control their substrate specificity or subcellular localization. Plants harbor several PP1 isoforms accounting for large functional redundancies. While animal PP1s were reported to play relevant roles in controlling multiple cellular processes, plant orthologs remain poorly studied. To decipher the role of plant PP1s, we compared PP1 genes from three monocot species, Brachypodium, common wheat and rice at the genomic and transcriptomic levels. To gain more insight into the wheat PP1 proteins, we identified and characterized TdPP1a, the first wheat type one protein phosphatase from a Tunisian durum wheat variety Oum Rabiaa3. TdPP1a is highly conserved in sequence and structure when compared to mammalian, yeast and other plant PP1s. We demonstrate that TdPP1a is an active, metallo-dependent phosphatase in vitro and is able to interact with AtI2, a typical regulator of PP1 functions. Also, TdPP1a is capable to complement the heat stress sensitivity of the yeast mutant indicating that TdPP1a is functional also in vivo. Moreover, transient expression of TdPP1a::GFP in tobacco leaves revealed that it is ubiquitously distributed within the cell, with a strong accumulation in the nucleus. Finally, transcriptional analyses showed similar expression levels in roots and leaves of durum wheat seedlings. Interestingly, the expression in leaves is significantly induced following salinity stress, suggesting a potential role of TdPP1a in wheat salt stress response.