Dose enhancement effects of gold nanoparticles specifically targeting RNA in breast cancer cells

Localization microscopy has shown to be capable of systematic investigations on the arrangement and counting of cellular uptake of gold nanoparticles (GNP) with nanometer resolution. In this article, we show that the application of specially modified RNA targeting gold nanoparticles ("SmartFlar...

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Bibliographic Details
Published in:PloS one 2018-01, Vol.13 (1), p.e0190183-e0190183
Main Authors: Hildenbrand, Georg, Metzler, Philipp, Pilarczyk, Götz, Bobu, Vladimir, Kriz, Wilhelm, Hosser, Hiltraud, Fleckenstein, Jens, Krufczik, Matthias, Bestvater, Felix, Wenz, Frederik, Hausmann, Michael
Format: Article
Language:English
Subjects:
Astronomy
Biology and Life Sciences
Breast cancer
Cancer
Care and treatment
Cell cycle
Deoxyribonucleic acid
Development and progression
DNA
Electron microscopy
Engineering and Technology
Genetic aspects
Gold
Gold compounds
Health aspects
Innovations
Irradiation
Localization
Magnetic fields
Medical research
Medical technology
Membrane structures
Microscopy
Molecular targeted therapy
Nanoparticles
Nanotechnology
Nuclei
Nuclei (cytology)
Oncology
Open access publishing
Particle size
Physical Sciences
Physics
Radiation
Radiation therapy
Research and Analysis Methods
Ribonucleic acid
RNA
Transmission electron microscopy
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Summary:Localization microscopy has shown to be capable of systematic investigations on the arrangement and counting of cellular uptake of gold nanoparticles (GNP) with nanometer resolution. In this article, we show that the application of specially modified RNA targeting gold nanoparticles ("SmartFlares") can result in ring like shaped GNP arrangements around the cell nucleus. Transmission electron microscopy revealed GNP accumulation in vicinity to the intracellular membrane structures including them of the endoplasmatic reticulum. A quantification of the radio therapeutic dose enhancement as a proof of principle was conducted with γH2AX foci analysis: The application of both-SmartFlares and unmodified GNPs-lead to a significant dose enhancement with a factor of up to 1.2 times the dose deposition compared to non-treated breast cancer cells. This enhancement effect was even more pronounced for SmartFlares. Furthermore, it was shown that a magnetic field of 1 Tesla simultaneously applied during irradiation has no detectable influence on neither the structure nor the dose enhancement dealt by gold nanoparticles.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0190183