Soluble factors from adipose tissue-derived mesenchymal stem cells promote canine hepatocellular carcinoma cell proliferation and invasion

• Published in: PloS one 2018-01, Vol.13 (1), p.e0191539-e0191539
• Main Authors: Teshima, Takahiro, Matsumoto, Hirotaka, Koyama, Hidekazu
• Format: Article
• Language: English
• Subjects: Adipose tissue; Antibiotics; Biology and Life Sciences; Body fat; Cell growth; Cell proliferation; Clinical medicine; Conditioning; Development and progression; Epidermal growth factor; Flow cytometry; Gelatinase A; Gelatinase B; Gene expression; Genetic aspects; Growth factors; Hepatocellular carcinoma; Hepatocyte growth factor; Insulin; Internal medicine; Laboratories; Life sciences; Liver cancer; Medicine and Health Sciences; Mesenchymal stem cells; Mesenchyme; Physiological aspects; Platelet-derived growth factor; Research and Analysis Methods; Rodents; Stem cells; Transforming growth factor-b1; Tumors; Vascular endothelial growth factor; Veterinary colleges; Veterinary medicine
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0191539

The potential effects of adipose tissue-derived mesenchymal stem cells (AT-MSCs) on the growth and invasion of canine tumours including hepatocellular carcinoma (HCC) are not yet understood. Moreover in humans, the functional contribution of AT-MSCs to malignancies remains controversial. The purpose of this study was to investigate the effects of AT-MSCs on the proliferation and invasion of canine HCC cells in vitro. The effect of AT-MSCs on mRNA levels of factors related to HCC progression were also evaluated. Conditioned medium from AT-MSCs (AT-MSC-CM) significantly enhanced canine HCC cell proliferation and invasion. Moreover, mRNA expression levels of transforming growth factor-beta 1, epidermal growth factor A, hepatocyte growth factor, platelet-derived growth factor-beta, vascular endothelial growth factor, and insulin-like growth factor 2 were 2.3 ± 0.4, 2.0 ± 0.5, 5.7 ± 1.9, 1.7 ± 0.2, 2.1 ± 0.4, and 1.4 ± 0.3 times higher, respectively (P < 0.05). The mRNA expression level of MMP-2 also increased (to 4.0 ± 1.2 times control levels) in canine HCC cells co-cultured with AT-MSCs, but MMP-9 mRNA significantly decreased (to 0.5 ± 0.1 times control levels). These findings suggest that soluble factors from AT-MSCs promote the proliferation and invasion of canine HCC cells.