Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin

• Published in: PloS one 2018-01, Vol.13 (1), p.e0191686
• Main Authors: Yang, Yajun, Xu, Hongxing, Lu, Yanhui, Wang, Caiyun, Lu, Zhongxian
• Format: Article
• Language: English
• Subjects: ABC transporter; Antigen presentation; Antigen processing; Antigens; Apoptosis; Bacillus thuringiensis; Biology and Life Sciences; Carboxylesterase; Carboxypeptidase; Chronic myeloid leukemia; Chymotrypsin; Comparative analysis; Crop diseases; CRY protein; Cry1C toxin; Detoxification; Disease control; Gene expression; Gene sequencing; Genes; Glutathione; Glutathione transferase; Helicoverpa armigera; Immunological tolerance; Insect pests; Insecticides; Insects; Laboratories; Larvae; Lerodea eufala; Leukemia; Medicine and Health Sciences; Midgut; Mutation; Myeloid leukemia; Oryza; Pest control; Pests; Proteins; Proteolysis; Research and Analysis Methods; Ribonucleic acid; Rice; RNA; Serine; Serine proteinase; Toxicity; Transporter; Trypsin
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0191686

Cnaphalocrocis medinalis (Guenée) is one of the important insect pests in rice field. Bt agents were recommended in the C. medinalis control and Bt rice is bred as a tactic to control this insect. However, the tolerance or resistance of insect to Bt protein is a main threat to the application of Bt protein. In order to investigate the response of C. medinalis transcriptome in defending a Cry1C toxin, high-through RNA-sequencing was carried in the C. medinalis larvae treated with and without Cry1C toxin. A total of 35,586 high-quality unigenes was annotated in the transcriptome of C. medinalis midgut. The comparative analysis identified 6,966 differently expressed unigenes (DEGs) between the two treatments. GO analysis showed that these genes involved in proteolysis and extracellular region. Among these DEGs, carboxylesterase, glutathione S-transferase and P450 were differently expressed in the treated C. medinalis midgut. Furthermore, trypsin, chymotrypsin, and carboxypeptidase were identified in DEGs, and most of them up-regulated. In addition, thirteen ABC transporters were downregulated and three upregulated in Cry1C-treated C. medinalis midgut. Based on the pathway analysis, antigen processing and presentation pathway, and chronic myeloid leukemia pathway were significant in C. medinalis treated with Cry1C toxin. These results indicated that serine protease, detoxification enzymes and ABC transporter, antigen processing and presentation pathway, and chronic myeloid leukemia pathway may involved in the response of C. medinalis to Cry1C toxin. This study provides a transcriptomal foundation for the identification and functional characterization of genes involved in the toxicity of Bt Cry protein against C. medinalis, and provides potential clues to the studies on the tolerance or resistance of an agriculturally important insect pest C. medinalis to Cry1C toxin.