bmo-miR-275 down-regulates expression of Bombyx mori sericin gene 2 in vitro

We hypothesized that bmo-miR-275 has a potential regulatory function regarding the expression of sericin gene 2 (BmSer-2). First, we examined the expression of bmo-miR-275 and its target gene BmSer-2 in seven different tissues from 5th instar day-3 silkworm larvae. The results showed that they were...

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Bibliographic Details
Published in:PloS one 2018-01, Vol.13 (1), p.e0190464-e0190464
Main Authors: Qian, Ping, Jiang, Tao, Wang, Xin, Song, Fei, Chen, Chen, Shen, Xingjia
Format: Article
Language:English
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Agriculture
Bioinformatics
Biology and life sciences
Biotechnology
Bombyx mori
Gene expression
Genes
Genomics
IE1 protein
Laboratories
Larvae
MicroRNAs
miRNA
Molecular biology
Plasmids
Proteins
Research and Analysis Methods
Silk
Silk gland
Tissues
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Summary:We hypothesized that bmo-miR-275 has a potential regulatory function regarding the expression of sericin gene 2 (BmSer-2). First, we examined the expression of bmo-miR-275 and its target gene BmSer-2 in seven different tissues from 5th instar day-3 silkworm larvae. The results showed that they were both specifically expressed in the middle silk gland, implying that spatio-temporal conditions are required for bmo-miR-275 to regulate the expression of BmSer-2. To test this hypothesis, we constructed a pri-bmo-miR-275 expressing plasmid pcDNA3.0 [ie1-egfp-pri-bmo-miR-275-SV40] and BmSer-2-3´UTR recombinant reporter plasmids pGL3.0 [A3-luc-Ser-2-3' UTR-SV40]. Finally, BmN cells were harvested and luciferase activity was detected. Results showed that luciferase activity was reduced significantly (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0190464