The proteomic response of the reef coral Pocillopora acuta to experimentally elevated temperatures

• Published in: PloS one 2018-01, Vol.13 (1), p.e0192001-e0192001
• Main Authors: Mayfield, Anderson B, Chen, Yi-Jyun, Lu, Chi-Yu, Chen, Chii-Shiarng
• Format: Article
• Language: English
• Subjects: Acclimation; Acclimatization; Biology and Life Sciences; Cellular stress response; Compartments; Coral Reefs; Corals; Cytoskeleton; Dinoflagellates; Earth Sciences; Electrophoresis, Gel, Two-Dimensional; Gels; Gene expression; High temperature; Homeostasis; Immune response; Immunity; Kinases; Mass Spectrometry; Mass spectroscopy; Medical research; Metabolism; Microorganisms; Mutualism; Physiological aspects; Pocillopora; Pocillopora damicornis; Pocilloporidae; Proteins; Proteomics; Seriatopora hystrix; Stress (Physiology); Symbiodinium; Temperature; Temperature effects; Temperature rise; Upwelling
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0192001

Although most reef-building corals live near the upper threshold of their thermotolerance, some scleractinians are resilient to temperature increases. For instance, Pocillopora acuta specimens from an upwelling habitat in Southern Taiwan survived a nine-month experimental exposure to 30°C, a temperature hypothesized to induce stress. To gain a greater understanding of the molecular pathways underlying such high-temperature acclimation, the protein profiles of experimental controls incubated at 27°C were compared to those of conspecific P. acuta specimens exposed to 30°C for two, four, or eight weeks, and differentially concentrated proteins (DCPs) were removed from the gels and sequenced with mass spectrometry. Sixty unique DCPs were uncovered across both eukaryotic compartments of the P. acuta-dinoflagellate (genus Symbiodinium) mutualism, and Symbiodinium were more responsive to high temperature at the protein-level than the coral hosts in which they resided at the two-week sampling time. Furthermore, proteins involved in the stress response were more likely to be documented at different cellular concentrations across temperature treatments in Symbiodinium, whereas the temperature-sensitive host coral proteome featured numerous proteins involved in cytoskeletal structure, immunity, and metabolism. These proteome-scale data suggest that the coral host and its intracellular dinoflagellates have differing strategies for acclimating to elevated temperatures.