Adaptability of the Saccharomyces cerevisiae yeasts to wine fermentation conditions relies on their strong ability to consume nitrogen

• Published in: PloS one 2018-02, Vol.13 (2), p.e0192383-e0192383
• Main Authors: Brice, Claire, Cubillos, Francisco A, Dequin, Sylvie, Camarasa, Carole, Martínez, Claudio
• Format: Article
• Language: English
• Subjects: Adaptability; Amino acids; Biology and Life Sciences; Cellular stress response; Chemical research; Domestication; Ecological niches; Evolution & development; Fermentation; Genes; Genetic diversity; Life Sciences; Medicine and Health Sciences; Metabolism; Microbiological synthesis; Microbiology and Parasitology; Niches; Nitrogen; Nitrogen (Chemical element); Phenotypic variations; Physical Sciences; Physiological aspects; Production processes; Research and Analysis Methods; Saccharomyces; Saccharomyces cerevisiae; Signal transduction; Signaling; Wine; Wines; Yeast; Yeasts
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0192383

Saccharomyces cerevisiae strains are genetically diverse, largely as a result of human efforts to develop strains specifically adapted to various fermentation processes. These adaptive pressures from various ecological niches have generated behavioral differences among these strains, particularly in terms of their nitrogen consumption capacities. In this work, we characterize this phenotype by the specific quantity of nitrogen consumed under oenological fermentation conditions using a new approach. Indeed, unlike previous studies, our experiments were conducted in an environment containing excess nitrogen, eliminating the nitrogen limitation/starvation factor that is generally observed in fermentation processes. Using these conditions, we evaluated differences in the nitrogen consumption capacities for a set of five strains from diverse origins. The strains presented extremely different phenotypes and variations in their capacities to take up nitrogen from a wine fermentation environment. These variations reflect the differences in the nitrogen uptake capacities between wine and non-wine strains. Finally, the strains differed in their ability to adapt to the nitrogen composition of the environment, leading to variations in the cellular stress states, fermentation performances and the activity of the nitrogen sensing signaling pathway.