A tissue-specific scaffold for tissue engineering-based ureteral reconstruction

Terminally differentiated somatic cells can rapidly change phenotypes when they are isolated from their native tissue and cultured in vitro. This problem may become a barrier to tissue engineering-based organ reconstruction, which utilizes somatic cells. The present study was designed to validate th...

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Bibliographic Details
Published in:PloS one 2015-03, Vol.10 (3), p.e0120244-e0120244
Main Authors: Xu, Yongde, Fu, Weijun, Wang, Zhongxin, Li, Gang, Zhang, Xu
Format: Article
Language:English
Subjects:
Adhesion tests
Adult
Biomarkers - metabolism
Biomedical materials
Cell adhesion
Cell adhesion & migration
Cell growth
Cell Proliferation
Cell Survival
Engineering
Extracellular matrix
Extracellular Matrix - metabolism
Feasibility studies
Guided Tissue Regeneration - methods
Humans
Intestine
Lactic Acid
Mechanical properties
Middle Aged
Mucosa
Organic acids
Permeability
Phenotype
Phenotypes
Polyesters
Polylactic acid
Polymers
Reconstruction
Somatic cells
Substrates
Tissue engineering
Tissue Engineering - methods
Tissue Scaffolds
Ureter
Ureter - cytology
Ureter - metabolism
Urogenital system
Urology
Urothelium
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Summary:Terminally differentiated somatic cells can rapidly change phenotypes when they are isolated from their native tissue and cultured in vitro. This problem may become a barrier to tissue engineering-based organ reconstruction, which utilizes somatic cells. The present study was designed to validate the feasibility of maintaining the urothelial cell phenotype in a tissue-specific ureteral scaffold. The tissue-specific scaffold was fabricated by blending poly (L-lactic acid) (PLLA) and ureteral extracellular matrix (UECM) using electrostatic spinning technology. PLLA was used to enhance the mechanical properties, and UECM was used to mimic the natural components of the ureter. Primary urothelial cells (UCs), derived from ureteral mucosa, were seeded onto the tissue-specific scaffold to assess cell adhesion, proliferation and phenotypes at designated time points. The results showed that UCs in the tissue-specific scaffold exhibited better proliferation compared to cells in pure PLLA or a PLLA-small intestinal submucosa (PLLA-SIS) scaffold (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0120244