Comparison of two cannulation methods for assessment of intracavernosal pressure in a rat model

Intracavernous pressure (ICP) measurement is a well-established technique for assessing the erectile function, which was performed by cannulating either crus or shaft of the penis. However, there are no studies concerning the experimental performance of the two cannulation sites yet. The aim of this...

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Bibliographic Details
Published in:PloS one 2018-02, Vol.13 (2), p.e0193543-e0193543
Main Authors: Zhao, Shankun, Kang, Ran, Deng, Tuo, Luo, Lianmin, Wang, Jiamin, Li, Ermao, Luo, Jintai, Liu, Luhao, Wan, ShawPong, Zhao, Zhigang
Format: Article
Language:English
Subjects:
Androgens
Animal models
Biology and Life Sciences
Cannulation
Cardiovascular tests
Castration
Construction
Diagnosis
Erectile dysfunction
Hospitals
Impotence
Laboratory animals
Laparoscopy
Mathematical models
Medical examination
Medicine and Health Sciences
Methods
Morphology
Penis
Pressure
Rats
Research and Analysis Methods
Rodents
Studies
Subgroups
Surgery
Testosterone
Urology
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Summary:Intracavernous pressure (ICP) measurement is a well-established technique for assessing the erectile function, which was performed by cannulating either crus or shaft of the penis. However, there are no studies concerning the experimental performance of the two cannulation sites yet. The aim of this study was to compare the measuring outcomes using two different cannulation sites. To validate the capacity of our study, both normal and the castration-induced erectile dysfunction rat models were conducted. Fifty adult male Sprague-Dawley rats were randomized equally into two groups: an intact group and a castration group. Five rats from each group firstly underwent different stimulation parameters to detect the optimal erectile responses. The residual rats in each group were further assigned into two subgroups (n = 10 per subgroup) according to two different cannulation sites (crus or shaft of the corpus cavernosum). The ICP values were compared between groups after different interventions. The optimal parameters for mean maximum ICP were recorded at 2.5V and a frequency of 15 Hz. The rats under the two different cannulation sites tended to show similar ICP values in both the intact and the castration groups. However, the success rate in monitoring ICP was significantly higher in the groups cannulating into the shaft of the penis compared to the crus (100% vs. 70%; P = 0.02). Our data suggested that the method of cannulation into the penile shaft could serve as a better alternative for the ICP measurement in rats.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0193543