Comprehensive analysis of small RNAs expressed in developing male strobili of Cryptomeria japonica

Deep sequencing of small RNAs (sRNAs) in developing male strobili of second-generation offspring originating from a nuclear genic male sterile tree of Cryptomeria japonica were performed to characterize sRNA populations in the male strobili at early pollen developmental stages. Comparing to sequence...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2018-03, Vol.13 (3), p.e0193665-e0193665
Main Authors: Ujino-Ihara, Tokuko, Ueno, Saneyoshi, Uchiyama, Kentaro, Futamura, Norihiro
Format: Article
Language:English
Subjects:
Arabidopsis
Biology and life sciences
Biotechnology
Cloning
Cryptomeria japonica
Cupressaceae
Developmental stages
Forest products
Forestry
Gene expression
Genetic aspects
Genomes
Genomics
Medicine and Health Sciences
MicroRNAs
miRNA
Offspring
Organs
Oryza
Plant reproduction
Plant species
Plants
Pollen
Properties
Reproductive organs
Research and analysis methods
Ribonucleic acid
RNA
Species
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Deep sequencing of small RNAs (sRNAs) in developing male strobili of second-generation offspring originating from a nuclear genic male sterile tree of Cryptomeria japonica were performed to characterize sRNA populations in the male strobili at early pollen developmental stages. Comparing to sequences of microRNA (miRNA) families of plant species and sRNAs expressed in the reproductive organs of representative vascular plants, 37 conserved miRNA families were detected, of which eight were ubiquitously expressed in the reproductive organs of land plant species. In contrast, miR1083 was common in male reproductive organs of gymnosperm species but absent in angiosperm species. In addition to conserved miRNAs, 199 novel miRNAs candidates were predicted. The expression patterns of the obtained sRNAs were further investigated to detect the differentially expressed (DE) sRNAs between genic male sterile and fertile individuals. A total of 969 DE sRNAs were obtained and only three known miRNA families were included among them. These results suggest that both conserved and species-specific sRNAs contribute to the development of male strobili in C. japonica.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0193665