Genetic diversity of drug and multidrug-resistant Mycobacterium tuberculosis circulating in Veracruz, Mexico

Mexico is one of the most important contributors of drug and multidrug-resistant tuberculosis in Latin America; however, knowledge of the genetic diversity of drug-resistant tuberculosis isolates is limited. In this study, the genetic structure of 112 Mycobacterium tuberculosis strains from the sout...

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Published in:PloS one 2018-03, Vol.13 (3), p.e0193626-e0193626
Main Authors: Munro-Rojas, Daniela, Fernandez-Morales, Esdras, Zarrabal-Meza, José, Martínez-Cazares, Ma Teresa, Parissi-Crivelli, Aurora, Fuentes-Domínguez, Javier, Séraphin, Marie Nancy, Lauzardo, Michael, González-Y-Merchand, Jorge Alberto, Rivera-Gutierrez, Sandra, Zenteno-Cuevas, Roberto
Format: Article
Language:English
Subjects:
Antibiotics
Biodiversity
Biology and Life Sciences
Cluster analysis
Deoxyribonucleic acid
DNA
Drug resistance
Earth Sciences
Ecology and Environmental Sciences
Epidemiology
Genetic aspects
Genetic diversity
Genetic structure
Genotypes
Infectious diseases
Laboratories
Medicine
Medicine and Health Sciences
Multiculturalism & pluralism
Multidrug resistance
Multidrug resistant organisms
Mutation
Mycobacterium tuberculosis
People and places
Physical Sciences
Population
Resistance factors
Risk factors
Spoligotyping
Strains (organisms)
Tuberculosis
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creator Munro-Rojas, Daniela
Fernandez-Morales, Esdras
Zarrabal-Meza, José
Martínez-Cazares, Ma Teresa
Parissi-Crivelli, Aurora
Fuentes-Domínguez, Javier
Séraphin, Marie Nancy
Lauzardo, Michael
González-Y-Merchand, Jorge Alberto
Rivera-Gutierrez, Sandra
Zenteno-Cuevas, Roberto
description Mexico is one of the most important contributors of drug and multidrug-resistant tuberculosis in Latin America; however, knowledge of the genetic diversity of drug-resistant tuberculosis isolates is limited. In this study, the genetic structure of 112 Mycobacterium tuberculosis strains from the southeastern Mexico was determined by spoligotyping and 24-loci MIRU-VNTRs. The results show eight major lineages, the most of which was T1 (24%), followed by LAM (16%) and H (15%). A total of 29 (25%) isolates were identified as orphan. The most abundant SITs were SIT53/T1 and SIT42/LAM9 with 10 isolates each and SIT50/H3 with eight isolates. Fifty-two spoligotype patterns, twenty-seven clusters and ten clonal complexes were observed, demonstrating an important genetic diversity of drug and multidrug-resistant tuberculosis isolates in circulation and transmission level of these aggravated forms of tuberculosis. Being defined as orphan or as part of an orphan cluster, was a risk factor for multidrug resistant-tuberculosis (OR 2.5, IC 1.05-5.86 and OR 3.3, IC 1-11.03, respectively). Multiple correspondence analyses showed association of some clusters and SITs with specific geographical locations. Our study provides one of the most detailed description of the genetic structure of drug and multidrug-resistant tuberculosis strains in southeast Mexico, establishing for the first time a baseline of the genotypes observed in resistant isolates circulating, however further studies are required to better elucidate the genetic structure of tuberculosis in region and the factors that could be participating in their dispersion.
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1932-6203
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source Publicly Available Content Database; PubMed Central
subjects Antibiotics
Biodiversity
Biology and Life Sciences
Cluster analysis
Deoxyribonucleic acid
DNA
Drug resistance
Earth Sciences
Ecology and Environmental Sciences
Epidemiology
Genetic aspects
Genetic diversity
Genetic structure
Genotypes
Infectious diseases
Laboratories
Medicine
Medicine and Health Sciences
Multiculturalism & pluralism
Multidrug resistance
Multidrug resistant organisms
Mutation
Mycobacterium tuberculosis
People and places
Physical Sciences
Population
Resistance factors
Risk factors
Spoligotyping
Strains (organisms)
Tuberculosis
title Genetic diversity of drug and multidrug-resistant Mycobacterium tuberculosis circulating in Veracruz, Mexico
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