Foliar nitrogen metabolism of adult Douglas-fir trees is affected by soil water availability and varies little among provenances

• Published in: PloS one 2018-03, Vol.13 (3), p.e0194684-e0194684
• Main Authors: Du, Baoguo, Kreuzwieser, Jürgen, Dannenmann, Michael, Junker, Laura Verena, Kleiber, Anita, Hess, Moritz, Jansen, Kirstin, Eiblmeier, Monika, Gessler, Arthur, Kohnle, Ulrich, Ensminger, Ingo, Rennenberg, Heinz, Wildhagen, Henning
• Format: Article
• Language: English
• Subjects: Acclimatization; Adaptation; Adaptation, Physiological; Amino acids; Analysis; Arginine; Asparagine; Availability; Biogeochemistry; Biology and Life Sciences; Carbon dioxide; Climate; Climatic conditions; Coastal environments; Coastal waters; Coniferous forests; Coniferous trees; Douglas fir; Earth Sciences; Ecology; Ecosystem; Ecosystem biology; Ecosystems; Environmental conditions; Environmental effects; Environmental factors; Evolutionary biology; Fir trees; Forests; Gardens & gardening; Geography; Germany; Indoor environments; Metabolism; Moisture content; Nitrogen; Nitrogen - metabolism; North America; Physical Sciences; Physiological aspects; Physiology; Picea abies; Picea glauca; Pinus sylvestris; Plant Leaves - metabolism; Pools; Provenance; Pseudotsuga - growth & development; Pseudotsuga - metabolism; Pseudotsuga menziesii; Research and Analysis Methods; Soil - chemistry; Soil moisture; Soil water; Trees; Trees - growth & development; Trees - metabolism; Water - analysis; Water - metabolism; Water availability; Water content
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0194684

The coniferous forest tree Douglas-fir (Pseudotsuga menziesii) is native to the pacific North America, and is increasingly planted in temperate regions worldwide. Nitrogen (N) metabolism is of great importance for growth, resistance and resilience of trees. In the present study, foliar N metabolism of adult trees of three coastal and one interior provenance of Douglas-fir grown at two common gardens in southwestern Germany (Wiesloch, W; Schluchsee, S) were characterized in two subsequent years. Both the native North American habitats of the seed sources and the common garden sites in Germany differ in climate conditions. Total and mineral soil N as well as soil water content were higher in S compared to W. We hypothesized that i) provenances differ constitutively in N pool sizes and composition, ii) N pools are affected by environmental conditions, and iii) that effects of environmental factors on N pools differ among interior and coastal provenances. Soil water content strongly affected the concentrations of total N, soluble protein, total amino acids (TAA), arginine and glutamate. Foliar concentrations of total N, soluble protein, structural N and TAA of trees grown at W were much higher than in trees at S. Provenance effects were small but significant for total N and soluble protein content (interior provenance showed lowest concentrations), as well as arginine, asparagine and glutamate. Our data suggest that needle N status of adult Douglas-fir is independent from soil N availability and that low soil water availability induces a re-allocation of N from structural N to metabolic N pools. Small provenance effects on N pools suggest that local adaptation of Douglas-fir is not dominated by N conditions at the native habitats.