Chronic restraint stress induces excessive activation of primordial follicles in mice ovaries

Chronic stress is an important factor influencing people's health. It usually causes endocrinal disorders and a decline in reproduction in females. Although studies of both human and animals suggest a detrimental effect of stress on reproduction, the influence of chronic stress on the ovarian r...

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Published in:PloS one 2018-03, Vol.13 (3), p.e0194894-e0194894
Main Authors: Xu, Minhua, Sun, Junyan, Wang, Qian, Zhang, Qiuwan, Wei, Chunsheng, Lai, Dongmei
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
Activation
AKT protein
Biology and Life Sciences
Childrens health
Chromosome 10
Complications and side effects
Corticotropin-releasing hormone
Endocrinology
Females
Follicles
Genetic aspects
Homology
Hospitals
Kinases
Laboratory animals
Medicine
Medicine and Health Sciences
mRNA
Ovaries
Ovulation
Physiological aspects
Polymerase chain reaction
Psychological aspects
PTEN protein
Reproduction
Research and Analysis Methods
Rodents
Signaling
Social Sciences
Stress (Psychology)
Stresses
Studies
Tensin
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Sun, Junyan
Wang, Qian
Zhang, Qiuwan
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Lai, Dongmei
description Chronic stress is an important factor influencing people's health. It usually causes endocrinal disorders and a decline in reproduction in females. Although studies of both human and animals suggest a detrimental effect of stress on reproduction, the influence of chronic stress on the ovarian reservation and follicular development is still not clear. In this study, a chronic restraint stress (CRS) mouse model was used to investigate the effect of stress on ovarian reservation and follicular development and explore the underlying mechanism. In this study, after 8 weeks of CRS, primordial follicles were excessively activated in the ovaries of the CRS group compared with the control group. Further results showed that the activation of primordial follicles induced by CRS was involved in the increasing expression level of Kit ligand and its receptor Kit and the activation of phosphatidylinositol 3-kinase (PI3K)/phosphatase and tensin homolog deleted on chromosome 10 (PTEN)/protein kinase B (Akt) pathway. The corticotropin-releasing hormone (CRH) is a neuropeptide released due to stress, which plays an important role in regulating follicle development. A high level of serum CRH was detected in the CRS mouse model, and the real-time polymerase chain reaction assay showed that the mRNA level of its main receptor CRHR1increased in the ovaries of the CRS mouse group. Moreover, 100nM CRH significantly improved the activation of primordial follicles in newborn mouse ovaries in vitro. These results demonstrated that CRS could induce immoderate activation of primordial follicles accompanied by the activation of Kit-PI3K signaling, in which CRH might be an important endocrine factor.
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It usually causes endocrinal disorders and a decline in reproduction in females. Although studies of both human and animals suggest a detrimental effect of stress on reproduction, the influence of chronic stress on the ovarian reservation and follicular development is still not clear. In this study, a chronic restraint stress (CRS) mouse model was used to investigate the effect of stress on ovarian reservation and follicular development and explore the underlying mechanism. In this study, after 8 weeks of CRS, primordial follicles were excessively activated in the ovaries of the CRS group compared with the control group. Further results showed that the activation of primordial follicles induced by CRS was involved in the increasing expression level of Kit ligand and its receptor Kit and the activation of phosphatidylinositol 3-kinase (PI3K)/phosphatase and tensin homolog deleted on chromosome 10 (PTEN)/protein kinase B (Akt) pathway. The corticotropin-releasing hormone (CRH) is a neuropeptide released due to stress, which plays an important role in regulating follicle development. A high level of serum CRH was detected in the CRS mouse model, and the real-time polymerase chain reaction assay showed that the mRNA level of its main receptor CRHR1increased in the ovaries of the CRS mouse group. Moreover, 100nM CRH significantly improved the activation of primordial follicles in newborn mouse ovaries in vitro. 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subjects 1-Phosphatidylinositol 3-kinase
Activation
AKT protein
Biology and Life Sciences
Childrens health
Chromosome 10
Complications and side effects
Corticotropin-releasing hormone
Endocrinology
Females
Follicles
Genetic aspects
Homology
Hospitals
Kinases
Laboratory animals
Medicine
Medicine and Health Sciences
mRNA
Ovaries
Ovulation
Physiological aspects
Polymerase chain reaction
Psychological aspects
PTEN protein
Reproduction
Research and Analysis Methods
Rodents
Signaling
Social Sciences
Stress (Psychology)
Stresses
Studies
Tensin
title Chronic restraint stress induces excessive activation of primordial follicles in mice ovaries
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