Time-dependent regulation of morphological changes and cartilage differentiation markers in the mouse pubic symphysis during pregnancy and postpartum recovery

Animal models commonly serve as a bridge between in vitro experiments and clinical applications; however, few physiological processes in adult animals are sufficient to serve as proof-of-concept models for cartilage regeneration. Intriguingly, some rodents, such as young adult mice, undergo physiolo...

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Published in:PloS one 2018-04, Vol.13 (4), p.e0195304-e0195304
Main Authors: Castelucci, Bianca Gazieri, Consonni, Sílvio Roberto, Rosa, Viviane Souza, Sensiate, Lucimara Aparecida, Delatti, Paula Cristina Rugno, Alvares, Lúcia Elvira, Joazeiro, Paulo Pinto
Format: Article
Language:English
Subjects:
Animal models
Animal tissues
Animals
Arthritis
Biochemistry
Biocompatibility
Biology
Biology and Life Sciences
Biomedical materials
Bones
Cartilage
Cbfa-1 protein
Cells (biology)
Childbirth & labor
Collagen
Connective tissues
Differentiation
Doublecortin protein
Endocrinology
Fractures
Gene expression
Health aspects
Homeostasis
Markers
Medicine and Health Sciences
Morphology
Osteoprogenitor cells
Physiology
Postpartum
Pregnancy
Psychological aspects
Recovery
Recovery (Medical)
Regeneration
Restoration
Rodents
Sox9 protein
Tendons
Therapeutic applications
Time dependence
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cited_by cdi_FETCH-LOGICAL-c557t-65567c4b50ea81b223c5734c01231560597f393cf75d4013d71fe7d08faa99c13
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container_issue 4
container_start_page e0195304
container_title PloS one
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creator Castelucci, Bianca Gazieri
Consonni, Sílvio Roberto
Rosa, Viviane Souza
Sensiate, Lucimara Aparecida
Delatti, Paula Cristina Rugno
Alvares, Lúcia Elvira
Joazeiro, Paulo Pinto
description Animal models commonly serve as a bridge between in vitro experiments and clinical applications; however, few physiological processes in adult animals are sufficient to serve as proof-of-concept models for cartilage regeneration. Intriguingly, some rodents, such as young adult mice, undergo physiological connective tissue modifications to birth canal elements such as the pubic symphysis during pregnancy; therefore, we investigated whether the differential expression of cartilage differentiation markers is associated with cartilaginous tissue morphological modifications during these changes. Our results showed that osteochondral progenitor cells expressing Runx2, Sox9, Col2a1 and Dcx at the non-pregnant pubic symphysis proliferated and differentiated throughout pregnancy, giving rise to a complex osteoligamentous junction that attached the interpubic ligament to the pubic bones until labour occurred. After delivery, the recovery of pubic symphysis cartilaginous tissues was improved by the time-dependent expression of these chondrocytic lineage markers at the osteoligamentous junction. This process potentially recapitulates embryologic chondrocytic differentiation to successfully recover hyaline cartilaginous pads at 10 days postpartum. Therefore, we propose that this physiological phenomenon represents a proof-of-concept model for investigating the mechanisms involved in cartilage restoration in adult animals.
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1932-6203
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source Publicly Available Content Database; PubMed Central
subjects Animal models
Animal tissues
Animals
Arthritis
Biochemistry
Biocompatibility
Biology
Biology and Life Sciences
Biomedical materials
Bones
Cartilage
Cbfa-1 protein
Cells (biology)
Childbirth & labor
Collagen
Connective tissues
Differentiation
Doublecortin protein
Endocrinology
Fractures
Gene expression
Health aspects
Homeostasis
Markers
Medicine and Health Sciences
Morphology
Osteoprogenitor cells
Physiology
Postpartum
Pregnancy
Psychological aspects
Recovery
Recovery (Medical)
Regeneration
Restoration
Rodents
Sox9 protein
Tendons
Therapeutic applications
Time dependence
title Time-dependent regulation of morphological changes and cartilage differentiation markers in the mouse pubic symphysis during pregnancy and postpartum recovery
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