Metabolism and prebiotics activity of anthocyanins from black rice (Oryza sativa L.) in vitro

Anthocyanins are naturally active substances. In this study, anthocyanins from black rice were obtained by membrane filtration and column chromatography separation. Five anthocyanin monomers in black rice extract were identified by HPLC-MS/MS, and the major anthocyanin monomer (cyanidin-3-glucoside,...

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Bibliographic Details
Published in:PloS one 2018-04, Vol.13 (4), p.e0195754-e0195754
Main Authors: Zhu, Yongsheng, Sun, Hanju, He, Shudong, Lou, Qiuyan, Yu, Min, Tang, Mingming, Tu, Lijun
Format: Article
Language:English
Subjects:
Anthocyanins
Bacteria
Bifidobacterium bifidum
Biology and Life Sciences
Chemical properties
Chemical research
Chromatography
Column chromatography
Composition
Engineering
Food science
Health aspects
High performance liquid chromatography
Incubation
Lactobacillus
Liquid chromatography
Membrane filtration
Metabolism
Metabolites
Microbiota
Monomers
Oryza sativa
pH effects
Polyphenols
Probiotics
Research and Analysis Methods
Rice
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Summary:Anthocyanins are naturally active substances. In this study, anthocyanins from black rice were obtained by membrane filtration and column chromatography separation. Five anthocyanin monomers in black rice extract were identified by HPLC-MS/MS, and the major anthocyanin monomer (cyanidin-3-glucoside, C3G) was purified by preparative HPLC (Pre-HPLC). The proliferative effects of the anthocyanins on Bifidobacteria and Lactobacillus were investigated by determining the media pH, bacterial populations and metabolic products. After anaerobic incubation at 37 °C for 48 h, not only the pH of the media containing C3G was lower than that of the extract of black rice anthocyanin (BRAE), but the numbers of both Bifidobacteria and Lactobacillus were also significantly increased. Furthermore, hydroxyphenylpropionic, hydroxyphenylacetic, and hydroxybenzoic acids and other metabolites were detected by GC-MS in vitro. Our results revealed that the anthocyanins and anthocyanin monomers from black rice had prebiotic activity and they were metabolized into several small molecules by Bifidobacteria and Lactobacillus.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0195754