Antioxidant capacity of phenolics in Camellia nitidissima Chi flowers and their identification by HPLC Triple TOF MS/MS

Camellia nitidissima Chi (CNC) is a valuable medicinal and edible plant in China. In this study, CNC flowers were extracted with 95% ethanol, then partitioned into dichloromethane, ethyl acetate, n-butanol, and water fractions, with the antioxidant capacity of flavonoids and other phytochemicals in...

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Published in:PloS one 2018-04, Vol.13 (4), p.e0195508-e0195508
Main Authors: Yang, Rui, Guan, Ying, Wang, Weixin, Chen, Hongjuan, He, Zhaochun, Jia, Ai-Qun
Format: Article
Language:English
Subjects:
Acetic acid
Acids
Antioxidants
Bioengineering
Biology and Life Sciences
Butanol
Camellia
Camellia - chemistry
Camellias
Cardiovascular disease
Catechol
Chromatography
Chromatography, High Pressure Liquid
Correlation analysis
Correlation coefficient
Correlation coefficients
Dichloromethane
Engineering
Ethanol
Ethyl acetate
Flavonoids
Flowers
Flowers - chemistry
Food
Free Radical Scavengers - chemistry
Free radicals
Glycosides
High-performance liquid chromatography
Iron constituents
Iron sulfates
Laboratories
Liquid chromatography
Medicinal plants
NMR
Nuclear magnetic resonance
Numerical controls
Phenolic compounds
Phenolic resins
Phenols
Phenols - chemistry
Physical Sciences
Physiological aspects
Phytochemicals
Principal components analysis
Recovery (Medical)
Research and Analysis Methods
Seeds
Steric hindrance
Tandem Mass Spectrometry
Tea
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creator Yang, Rui
Guan, Ying
Wang, Weixin
Chen, Hongjuan
He, Zhaochun
Jia, Ai-Qun
description Camellia nitidissima Chi (CNC) is a valuable medicinal and edible plant in China. In this study, CNC flowers were extracted with 95% ethanol, then partitioned into dichloromethane, ethyl acetate, n-butanol, and water fractions, with the antioxidant capacity of flavonoids and other phytochemicals in CNC flowers investigated for the first time. Results showed that the ethyl acetate fraction exhibited the strongest antioxidant capacity and highest total phenolic content (TPC) compared with the other fractions. Furthermore, in the ethyl acetate fraction, the 50% effective concentrations (EC50) of ABTS+ and DPPH radical scavenging activities were 64.24 ± 1.80 and 78.80 ± 0.34 μg/mL, respectively, and the ferric reducing antioxidant power (FRAP) was 801.49 ± 2.30 μM FeSO4 at 1,000 μg/mL. Pearson's correlation coefficients and principal component analyses (PCA) for the TPC and antioxidant capacity of the five fractions indicated that the phenolic compounds were the major antioxidant constituents in the flowers. To exploit the antioxidants in CNC flowers, 21 phenolic compounds in the ethanolic extract fraction were identified by HPLC Triple TOF MS/MS, next, 12 flavonoids were isolated and elucidated, of which compounds 1-5 showed potent antioxidant capacity. In addition, the potential structure-activity relationship among these 12 flavonoids showed that (1) the o-catechol group in the B-ring was primarily responsible for the antioxidant capacity of flavonoids and (2) steric hindrance, produced by glycosides and other groups, could reduce the antioxidant capacity of the flavonoids.
doi_str_mv 10.1371/journal.pone.0195508
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In this study, CNC flowers were extracted with 95% ethanol, then partitioned into dichloromethane, ethyl acetate, n-butanol, and water fractions, with the antioxidant capacity of flavonoids and other phytochemicals in CNC flowers investigated for the first time. Results showed that the ethyl acetate fraction exhibited the strongest antioxidant capacity and highest total phenolic content (TPC) compared with the other fractions. Furthermore, in the ethyl acetate fraction, the 50% effective concentrations (EC50) of ABTS+ and DPPH radical scavenging activities were 64.24 ± 1.80 and 78.80 ± 0.34 μg/mL, respectively, and the ferric reducing antioxidant power (FRAP) was 801.49 ± 2.30 μM FeSO4 at 1,000 μg/mL. Pearson's correlation coefficients and principal component analyses (PCA) for the TPC and antioxidant capacity of the five fractions indicated that the phenolic compounds were the major antioxidant constituents in the flowers. To exploit the antioxidants in CNC flowers, 21 phenolic compounds in the ethanolic extract fraction were identified by HPLC Triple TOF MS/MS, next, 12 flavonoids were isolated and elucidated, of which compounds 1-5 showed potent antioxidant capacity. In addition, the potential structure-activity relationship among these 12 flavonoids showed that (1) the o-catechol group in the B-ring was primarily responsible for the antioxidant capacity of flavonoids and (2) steric hindrance, produced by glycosides and other groups, could reduce the antioxidant capacity of the flavonoids.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29634769</pmid><doi>10.1371/journal.pone.0195508</doi><tpages>e0195508</tpages><orcidid>https://orcid.org/0000-0002-8089-6200</orcidid><oa>free_for_read</oa></addata></record>
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subjects Acetic acid
Acids
Antioxidants
Bioengineering
Biology and Life Sciences
Butanol
Camellia
Camellia - chemistry
Camellias
Cardiovascular disease
Catechol
Chromatography
Chromatography, High Pressure Liquid
Correlation analysis
Correlation coefficient
Correlation coefficients
Dichloromethane
Engineering
Ethanol
Ethyl acetate
Flavonoids
Flowers
Flowers - chemistry
Food
Free Radical Scavengers - chemistry
Free radicals
Glycosides
High-performance liquid chromatography
Iron constituents
Iron sulfates
Laboratories
Liquid chromatography
Medicinal plants
NMR
Nuclear magnetic resonance
Numerical controls
Phenolic compounds
Phenolic resins
Phenols
Phenols - chemistry
Physical Sciences
Physiological aspects
Phytochemicals
Principal components analysis
Recovery (Medical)
Research and Analysis Methods
Seeds
Steric hindrance
Tandem Mass Spectrometry
Tea
title Antioxidant capacity of phenolics in Camellia nitidissima Chi flowers and their identification by HPLC Triple TOF MS/MS
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