Diversity of cultivable fungal endophytes in Paullinia cupana (Mart.) Ducke and bioactivity of their secondary metabolites

• Published in: PloS one 2018-04, Vol.13 (4), p.e0195874-e0195874
• Main Authors: Silva, Fábio de Azevedo, Liotti, Rhavena Graziela, Boleti, Ana Paula de Araújo, Reis, Érica de Melo, Passos, Marilene Borges Silva, Dos Santos, Edson Lucas, Sampaio, Olivia Moreira, Januário, Ana Helena, Branco, Carmen Lucia Bassi, Silva, Gilvan Ferreira da, Mendonça, Elisabeth Aparecida Furtado de, Soares, Marcos Antônio
• Format: Article
• Language: English
• Subjects: Acids; Animals; Anthracnose; Antibiotics; Anticancer properties; Biodiversity; Biological activity; Biology and Life Sciences; Biotechnology; CHO Cells; Cluster Analysis; Communities; Cricetulus; Crop diseases; Cytotoxicity; Earth Sciences; Ecology and Environmental Sciences; Endophytes; Fungi; Fungi - classification; Fungi - isolation & purification; Fungi - metabolism; Genotoxicity; Genotypes; Guarana (Plant); Host plants; Klebsiella pneumoniae; Medicine and Health Sciences; Metabolites; Metabolome; Metabolomics - methods; Microbiomes; Microorganisms; Paullinia - microbiology; Paullinia cupana; Physiological aspects; Plant diseases; Plant growth; Plant metabolites; Plant protection; Plant Roots - microbiology; Pseudomonas aeruginosa; Quantitative Trait, Heritable; Secondary Metabolism; Secondary metabolites; Seeds; Seeds - microbiology; Species; Staphylococcus infections; Strains (organisms); Streptomyces; VOCs; Volatile organic compounds
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0195874

Paullinia cupana is associated with a diverse community of pathogenic and endophytic microorganisms. We isolated and identified endophytic fungal communities from the roots and seeds of P. cupana genotypes susceptible and tolerant to anthracnose that grow in two sites of the Brazilian Amazonia forest. We assessed the antibacterial, antitumor and genotoxic activity in vitro of compounds isolated from the strains Trichoderma asperellum (1BDA) and Diaporthe phaseolorum (8S). In concert, we identified eight fungal species not previously reported as endophytes; some fungal species capable of inhibiting pathogen growth; and the production of antibiotics and compounds with bacteriostatic activity against Pseudomonas aeruginosa in both susceptible and multiresistant host strains. The plant genotype, geographic location and specially the organ influenced the composition of P. cupana endophytic fungal community. Together, our findings identify important functional roles of endophytic species found within the microbiome of P. cupana. This hypothesis requires experimental validation to propose management of this microbiome with the objective of promoting plant growth and protection.