Actin and myosin II modulate differentiation of pluripotent stem cells

Use of stem cell-based therapies in tissue engineering and regenerative medicine is hindered by efficient means of directed differentiation. For pluripotent stem cells, an initial critical differentiation event is specification to one of three germ lineages: endoderm, mesoderm, and ectoderm. Differe...

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Bibliographic Details
Published in:PloS one 2018-04, Vol.13 (4), p.e0195588-e0195588
Main Authors: Boraas, Liana C, Pineda, Emma T, Ahsan, Tabassum
Format: Article
Language:English
Subjects:
Actin
Antagonists
Biocompatibility
Biology and Life Sciences
Biomedical engineering
Biomedical materials
Cardiomyocytes
Cell cycle
Cloning
Cytoskeleton
Differentiation
Ectoderm
Embryo cells
Endoderm
Endothelial cells
Heart diseases
Hepatocytes
Liver
Mesoderm
Myosin
Osteocytes
Pancreas
Pluripotency
Polymerization
Regenerative medicine
Shear stress
Specifications
Stem cells
Tissue engineering
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Summary:Use of stem cell-based therapies in tissue engineering and regenerative medicine is hindered by efficient means of directed differentiation. For pluripotent stem cells, an initial critical differentiation event is specification to one of three germ lineages: endoderm, mesoderm, and ectoderm. Differentiation is known to be regulated by numerous extracellular and intracellular factors, but the role of the cytoskeleton during specification, or early differentiation, is still unknown. In these studies, we used agonists and antagonists to modulate actin polymerization and the actin-myosin molecular motor during spontaneous differentiation of embryonic stem cells in embryoid bodies. We found that inhibiting either actin polymerization or actin-myosin interactions led to a decrease in differentiation to the mesodermal lineage and an increase in differentiation to the endodermal lineage. Thus, targeting processes that regulate cytoskeletal tension may be effective in enhancing or inhibiting differentiation towards cells of the endodermal or mesodermal lineages, which include hepatocytes, islets, cardiomyocytes, endothelial cells, and osteocytes. Therefore, these fundamental findings demonstrate that modulation of the cytoskeleton may be useful in production for a range of cell-based therapies, including for liver, pancreatic, cardiac, vascular, and orthopedic applications.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0195588