Myocyte enhancer factor 2A promotes proliferation and its inhibition attenuates myogenic differentiation via myozenin 2 in bovine skeletal muscle myoblast

Myocyte enhancer factor 2A (MEF2A) is widely distributed in various tissues or organs and plays crucial roles in multiple biological processes. To examine the potential effects of MEF2A on skeletal muscle myoblast, the functional role of MFE2A in myoblast proliferation and differentiation was invest...

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Bibliographic Details
Published in:PloS one 2018-04, Vol.13 (4), p.e0196255-e0196255
Main Authors: Wang, Ya-Ning, Yang, Wu-Cai, Li, Pei-Wei, Wang, Hong-Bao, Zhang, Ying-Ying, Zan, Lin-Sen
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
Analysis
Animal sciences
Animals
Beef
Biological activity
Biological effects
Biology and Life Sciences
Bovidae
Breeding of animals
Cardiomyopathy
Cattle
Cell culture
Cell Cycle
Cell Differentiation
Cell Proliferation
Cyclin-dependent kinase 2
Differentiation
Flow Cytometry
Gene expression
Gene Expression Regulation
Genes
Kinases
Medicine and Health Sciences
MEF2 Transcription Factors - physiology
Muscle Development
Muscle function
Muscle Proteins - physiology
Muscle, Skeletal - physiology
Muscles
Musculoskeletal system
Myoblasts, Skeletal - physiology
Myoblasts, Skeletal - ultrastructure
Myogenesis
Organs
Penicillin
Polymerase chain reaction
Promoter Regions, Genetic
Promoters (Genetics)
Research and Analysis Methods
RNA, Small Interfering - metabolism
Skeletal muscle
Transcription factors
Zoology
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Summary:Myocyte enhancer factor 2A (MEF2A) is widely distributed in various tissues or organs and plays crucial roles in multiple biological processes. To examine the potential effects of MEF2A on skeletal muscle myoblast, the functional role of MFE2A in myoblast proliferation and differentiation was investigated. In this study, we found that the mRNA expression level of Mef2a was dramatically increased during the myogenesis of bovine skeletal muscle primary myoblast. Overexpression of MEF2A significantly promoted myoblast proliferation, while knockdown of MEF2A inhibited the proliferation and differentiation of myoblast. RT-PCR and western blot analysis revealed that this positive effect of MEF2A on the proliferation of myoblast was carried out by triggering cell cycle progression by activating CDK2 protein expression. Besides, MEF2A was found to be an important transcription factor that bound to the myozenin 2 (MyoZ2) proximal promoter and performed upstream of MyoZ2 during myoblast differentiation. This study provides the first experimental evidence that MEF2A is a positive regulator in skeletal muscle myoblast proliferation and suggests that MEF2A regulates myoblast differentiation via regulating MyoZ2.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0196255