Asymmetric polar localization dynamics of the serine chemoreceptor protein Tsr in Escherichia coli

The spatial location of proteins in living cells can be critical for their function. For example, the E. coli chemotaxis machinery is localized to the cell poles. Here we describe the polar localization of the serine chemoreceptor Tsr using a strain synthesizing a fluorescent Tsr-Venus fusion at a l...

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Bibliographic Details
Published in:PloS one 2018-05, Vol.13 (5), p.e0195887-e0195887
Main Authors: Oh, Dongmyung, Yu, Yang, Lee, Hochan, Jeon, Jae-Hyung, Wanner, Barry L, Ritchie, Ken
Format: Article
Language:English
Subjects:
Astronomy
Bacteria
Biology and Life Sciences
Cell cycle
Cell division
Chemotaxis
Chromosomes
Clusters
E coli
Escherichia coli
Fluorescence
Gene expression
Kinases
Localization
Low level
Molecular chains
Photobleaching
Physical Sciences
Physics
Polar wandering
Proteins
Recovery (Medical)
Research and Analysis Methods
Serine
Shigella
Synthesis
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Summary:The spatial location of proteins in living cells can be critical for their function. For example, the E. coli chemotaxis machinery is localized to the cell poles. Here we describe the polar localization of the serine chemoreceptor Tsr using a strain synthesizing a fluorescent Tsr-Venus fusion at a low level from a single-copy chromosomal construct. Using photobleaching and imaging during recovery by new synthesis, we observed distinct asymmetry between a bright (old) pole and a dim (new) pole. The old pole was shown to be a more stable cluster and to recover after photobleaching faster, which is consistent with the hypothesis that newly synthesized Tsr proteins are inserted directly at or near the old pole. The new pole was shown to be a less stable cluster and to exchange proteins freely with highly mobile Tsr-Venus proteins diffusing in the membrane. We propose that the new pole arises from molecules escaping from the old pole and diffusing to the new pole where a more stable cluster forms over time. Our localization imaging data support a model in which a nascent new pole forms prior to stable cluster formation.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0195887