Analysis of microRNAs in familial Mediterranean fever

Although Familial Mediterranean fever (FMF) is categorized as autosomal recessive, frequent exceptions to this model exist and therefore we aimed to search epigenetic modifications in this disease. Ten M694V homozygous FMF patients (the most severe phenotype) were recruited for this study. Patients...

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Bibliographic Details
Published in:PloS one 2018-05, Vol.13 (5), p.e0197829-e0197829
Main Authors: Amarilyo, Gil, Pillar, Nir, Ben-Zvi, Ilan, Weissglas-Volkov, Daphna, Zalcman, Jonatan, Harel, Liora, Livneh, Avi, Shomron, Noam
Format: Article
Language:English
Subjects:
Arthritis
Biology and life sciences
Biomarkers
Care and treatment
Cyclin-dependent kinases
Development and progression
DNA probes
Epigenetics
Familial Mediterranean fever
Fever
Gene expression
Gene mutation
Genetic aspects
Genotype & phenotype
Health aspects
Inflammation
Inflammatory diseases
Internal medicine
Kinases
Medicine
Medicine and Health Sciences
MicroRNA
MicroRNAs
miRNA
Mutation
Pathogenesis
Patients
Pediatrics
Periodic disease
Peripheral blood
Phenotypes
Physical Sciences
Research and Analysis Methods
Rheumatology
Ribonucleic acid
RNA
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Summary:Although Familial Mediterranean fever (FMF) is categorized as autosomal recessive, frequent exceptions to this model exist and therefore we aimed to search epigenetic modifications in this disease. Ten M694V homozygous FMF patients (the most severe phenotype) were recruited for this study. Patients with inflammatory flare were excluded. Total RNA was extracted from peripheral blood, and microRNA expression profiled using NanoString nCounter technology. These patients were compared to 10 healthy age- and sex-matched controls. Seven hundred nighty-eight mature human miRNAs were probed, 103 of which had expression levels above the negative control probes. Seven miRNAs showed significant differences in expression in samples from FMF patients compared to healthy controls: four miRNAs were upregulated (miR-144-3p, miR-21-5p, miR-4454, and miR-451a), and three were downregulated (miR-107, let-7d-5p, and miR-148b-3p). In this pilot study, we identified epigenetic modifications in clinically quiescent FMF patients. More studies are required for exploration of their contribution to FMF pathogenesis and their potential role as clinical biomarkers.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0197829