Characterization of the murine orthotopic adamantinomatous craniopharyngioma PDX model by MRI in correlation with histology

Adamantinomatous craniopharyngiomas (ACP) as benign sellar brain tumors are challenging to treat. In order to develop robust in vivo drug testing methodology, the murine orthotopic craniopharyngioma model (PDX) was characterized by magnetic resonance imaging (MRI) and histology in xenografts from th...

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Bibliographic Details
Published in:PloS one 2018-05, Vol.13 (5), p.e0197895-e0197895
Main Authors: Hölsken, Annett, Schwarz, Marc, Gillmann, Clarissa, Pfister, Christina, Uder, Michael, Doerfler, Arnd, Buchfelder, Michael, Schlaffer, Sven, Fahlbusch, Rudolf, Buslei, Rolf, Bäuerle, Tobias
Format: Article
Language:English
Subjects:
Adult
Animals
Biology and Life Sciences
Bone cancer
Brain
Brain cancer
Brain tumors
Chemotherapy
Child
Child, Preschool
Contrast Media - metabolism
Craniopharyngioma - metabolism
Craniopharyngioma - pathology
Diffusion
Diffusion coefficient
Female
Grafts
Histology
Humans
In vivo methods and tests
Keratin
Magnetic resonance
Magnetic resonance imaging
Magnetic Resonance Imaging - methods
Male
Medical imaging
Medicine and Health Sciences
Mice
Middle Aged
Morphology
Neoplasia
Neuroimaging
Neuropathology
Neurosurgery
Parameters
Patients
Phenotypes
Physical characteristics
Pituitary
Pituitary tumors
Qualitative analysis
Research and Analysis Methods
Solid tumors
Transplantation, Heterologous
Tumors
Xenografts
Xenotransplantation
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Summary:Adamantinomatous craniopharyngiomas (ACP) as benign sellar brain tumors are challenging to treat. In order to develop robust in vivo drug testing methodology, the murine orthotopic craniopharyngioma model (PDX) was characterized by magnetic resonance imaging (MRI) and histology in xenografts from three patients (ACP1-3). In ACP PDX, multiparametric MRI was conducted to assess morphologic characteristics such as contrast-enhancing tumor volume (CETV) as well as functional parameters from dynamic contrast-enhanced MRI (DCE-MRI) and diffusion-weighted imaging (DWI) including area-under-the-curve (AUC), peak enhancement (PE), time-to-peak (TTP) and apparent diffusion coefficient (ADC). These MRI parameters evaluated in 27 ACP PDX were correlated to histological features and percentage of vital tumor cell content. Qualitative analysis of MRI and histology from PDX revealed a similar phenotype as seen in patients, although the MRI appearance in mice resulted in a more solid tumor growth than in humans. CETV were significantly higher in ACP2 xenografts relative to ACP1 and ACP3 which correspond to respective average vitality of 41%,
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0197895