Clinical, pathological, and molecular features of classical and L-type atypical-BSE in goats

• Published in: PloS one 2018-05, Vol.13 (5), p.e0198037-e0198037
• Main Authors: Vallino Costassa, Elena, D'Angelo, Antonio, Mazza, Maria, Meloni, Daniela, Baioni, Elisa, Maurella, Cristiana, Colussi, Silvia, Martinelli, Nicola, Lo Faro, Monica, Berrone, Elena, Favole, Alessandra, Crociara, Paola, Grifoni, Silvia, Gallo, Marina, Lombardi, Guerino, Iulini, Barbara, Casalone, Cristina, Corona, Cristiano
• Format: Article
• Language: English
• Subjects: Alopecia; Animals; Biology and Life Sciences; Bovine spongiform encephalopathy; Brain - metabolism; Brain - pathology; BSE; Cattle; Classification; Creutzfeldt-Jakob disease; Disease control; Encephalopathy, Bovine Spongiform - metabolism; Encephalopathy, Bovine Spongiform - pathology; Encephalopathy, Bovine Spongiform - transmission; Experimental infection; Goat Diseases - metabolism; Goat Diseases - pathology; Goat Diseases - transmission; Goats; Histology; Immunohistochemistry; Incubation; Lesions; Medicine and Health Sciences; Molecular chains; Nervous system; Pathology, Clinical; Physiological aspects; Proteins; PrPSc Proteins - metabolism; Public health; Research and Analysis Methods; Scrapie; Scrapie - metabolism; Scrapie - pathology; Scrapie - transmission; Sheep; Skin diseases; Stability; Survival analysis; Transmissible spongiform encephalopathy
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0198037

Monitoring of small ruminants for transmissible spongiform encephalopathies (TSEs) has recently become more relevant after two natural scrapie suspected cases of goats were found to be positive for classical BSE (C-BSE). C-BSE probably established itself in this species unrecognized, undermining disease control measures. This opens the possibility that TSEs in goats may remain an animal source for human prion diseases. Currently, there are no data regarding the natural presence of the atypical BSE in caprines. Here we report that C-BSE and L-type atypical BSE (L-BSE) isolates from bovine species are intracerebrally transmissible to goats, with a 100% attack rate and a significantly shorter incubation period and survival time after C-BSE than after L-BSE experimental infection, suggesting a lower species barrier for classical agentin goat. All animals showed nearly the same clinical features of disease characterized by skin lesions, including broken hair and alopecia, and abnormal mental status. Histology and immunohistochemistry showed several differences between C-BSE and L-BSE infection, allowing discrimination between the two different strains. The lymphoreticular involvement we observed in the C-BSE positive goats argues in favour of a peripheral distribution of PrPSc similar to classical scrapie. Western blot and other currently approved screening tests detected both strains in the goats and were able to classify negative control animals. These data demonstrate that active surveillance of small ruminants, as applied to fallen stock and/or healthy slaughter populations in European countries, is able to correctly identify and classify classical and L-BSE and ultimately protect public health.