Effects of medical ozone upon healthy equine joints: Clinical and laboratorial aspects

• Published in: PloS one 2018-05, Vol.13 (5), p.e0197736-e0197736
• Main Authors: Vendruscolo, Cynthia do Prado, Moreira, Juliana Junqueira, Seidel, Sarah Raphaela Torquato, Fülber, Joice, Neuenschwander, Henrique Macedo, Bonagura, Giancarlo, Agreste, Fernanda Rodrigues, Baccarin, Raquel Yvonne Arantes
• Format: Article
• Language: English
• Subjects: Acids; Animals; Antioxidants; Arthritis; Back pain; Bioindicators; Biology and Life Sciences; Biomarkers; Cartilage; Catabolism; Chondroitin sulfate; Chondroitin Sulfates - metabolism; Clinical medicine; Complications and side effects; Cytokines; Cytokines - metabolism; Degradation; Degradation products; Development and progression; Disease; Dosage and administration; Dose-Response Relationship, Drug; Earth Sciences; Ecology and Environmental Sciences; Effusion; Eicosanoids - metabolism; Enzymes; Evaluation; Extracellular matrix; Health services; Horse Diseases - chemically induced; Horse Diseases - diagnostic imaging; Horse Diseases - metabolism; Horses; Hyaluronic acid; Hyaluronic Acid - metabolism; Inflammation; Interleukin 10; Interleukin 6; Joint diseases; Joints - drug effects; Joints - metabolism; Lameness, Animal - chemically induced; Lameness, Animal - diagnostic imaging; Lameness, Animal - metabolism; Lipid peroxidation; Medicine and Health Sciences; Metabolism; Molecular chains; Molecular weight; Oxidative stress; Oxygen; Ozone; Ozone - administration & dosage; Ozone - pharmacology; Ozone concentration; Physical Sciences; Physiological aspects; Pollution monitoring; Prostaglandin E2; Proteins; Random Allocation; Research and Analysis Methods; Risk factors; Substance P; Sulfates; Superoxide dismutase; Synovial fluid; Synovial Fluid - drug effects; Synovial Fluid - metabolism; Ultrasonography - veterinary; Ultrasound; Veterinary colleges; Veterinary medicine
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0197736

The aim of this study was to verify whether transient inflammatory reactions induced by intra-articular medicinal ozone administration affect joint components, by in vivo evaluation of inflammatory (prostaglandin E2, Substance P, Interleukin-6, Interleukine-1, Tumor Necrosis Factor), anti-inflammatory (Interleukin-10) and oxidative (superoxide dismutase activity and oxidative burst) biomarkers and extracellular matrix degradation products (chondroitin sulphate and hyaluronic acid) in synovial fluid. The effects of medicinal ozone were analyzed at two ozone concentrations (groups A and B, 20 and 40 μg/ml, respectively), using oxygen-injected joints as controls (group C); each group received ten treatments (15 ml gas per treatment). Physical evaluation, evaluation of lameness, ultrasonography, and synovial fluid analysis were performed. All joints presented mild and transient effusion throughout the study. Group B exhibited the highest lameness score on day 14 (P