Leishmanicidal and fungicidal activity of lipases obtained from endophytic fungi extracts

• Published in: PloS one 2018-06, Vol.13 (6), p.e0196796-e0196796
• Main Authors: Alves, Daniela Ribeiro, Morais, Selene Maia de, Tomiotto-Pellissier, Fernanda, Vasconcelos, Fábio Roger, Freire, Francisco das Chagas Oliveira, Silva, Isaac Neto Goes da, Cataneo, Alan Henrique Depieri, Miranda-Sapla, Milena Menegazzo, Pinto, Gustavo Adolfo Saavedra, Conchon-Costa, Ivete, Noronha, Arlindo de Alencar Araripe, Pavanelli, Wander Rogério
• Format: Article
• Language: English
• Subjects: Animal sciences; Animals; Antifungal activity; Antifungal agents; Antifungal Agents - chemistry; Antifungal Agents - pharmacology; Antiprotozoal Agents - chemistry; Antiprotozoal Agents - pharmacology; Arthrodermataceae - growth & development; Aspergillus; Biodiversity; Biological activity; Biology and Life Sciences; Complex Mixtures - chemistry; Complex Mixtures - pharmacology; Cytotoxicity; Dermatophyte fungi; Dichotomophtora boerhaaviae; Dichotomophtora portulacae; Emericella - chemistry; Emericella - enzymology; Emericella nidulans; Endophytes; Enzymes; Fungal Proteins - chemistry; Fungal Proteins - pharmacology; Fungi; Fungicidal activity; Fungicides; Laboratories; Leishmania - growth & development; Leishmania amazonensis; Lipase; Lipase - chemistry; Lipase - pharmacology; Lipids; Lipolysis; Malassezia; Medicine and Health Sciences; Metabolism; Metabolites; Methods; Mice; Mice, Inbred BALB C; Microsporum canis; Molecular chains; Parasites; Parasitic diseases; Physiology; Proteins; Proteomics; Research and Analysis Methods; Seeds; Viability; Zoology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0196796

This work describes the production of lipases from endophytic fungi: Vermisporium-like, Emericella nidulans, Dichotomophtora portulacae and D. boerhaaviae and the biological activity against the dermatophyte fungi Malassezia sp and Microsporum canis and the parasite Leishmania amazonensis. All fungal enzymes extract showed lipolysis action in the media that contains long carbon chain lipids. The proteomic analysis of lipases exhibits several molecules mostly ranging in size from 220 to 20 kDa, with clear differences in protein profile's yield. All fungal enzymes were competent to eliminate promastigote forms of Leishmania amazonensis at 5 mg.mL-1. The antileishmanial activity of lipases from Vermisporium-like, E. nidulans, D. portulacae and D. boerhaaviae in amastigote forms, promoted the reduction in viability of 78.88, 39.65, 63.17 and 98.13%, with selectivity index of 19.56, 30.68, 18.09 and 20.99. In relation to antifungal activity, Dichothomophtora enzymes demonstrate best action with MFC of 14.65 μg.mL-1 against Malassezia sp and Microsporum canis, respectively. These results allow us to infer that lipases from entophytic fungi displays activity against dermatophyte fungi (Malassezia sp. and Microsporum canis) as well as Leishmania.