Rv1460, a SufR homologue, is a repressor of the suf operon in Mycobacterium tuberculosis

Iron-sulphur (Fe-S) clusters are ubiquitous co-factors which require multi-protein systems for their synthesis. In Mycobacterium tuberculosis, the Rv1460-Rv1461-Rv1462-Rv1463-csd-Rv1465-Rv1466 operon (suf operon) encodes the primary Fe-S cluster biogenesis system. The first gene in this operon, Rv14...

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Bibliographic Details
Published in:PloS one 2018-07, Vol.13 (7), p.e0200145-e0200145
Main Authors: Willemse, Danicke, Weber, Brandon, Masino, Laura, Warren, Robin M, Adinolfi, Salvatore, Pastore, Annalisa, Williams, Monique J
Format: Article
Language:English
Subjects:
Bacteria
Biology and Life Sciences
Biosynthesis
Chromosomes
Circular dichroism
Clusters
Councils
Cysteine
Deoxyribonucleic acid
Dichroism
DNA
E coli
Enzymes
Escherichia coli
Gene expression
Genomes
Gram-positive bacteria
Health sciences
Homology
Infections
Iron
Medical research
Metabolism
Molecular biology
Mutants
Mycobacterium tuberculosis
Oxidative stress
Physical Sciences
Protein biosynthesis
Proteins
Spectroscopy
Sulfur
Supervision
Transcription
Tuberculosis
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Summary:Iron-sulphur (Fe-S) clusters are ubiquitous co-factors which require multi-protein systems for their synthesis. In Mycobacterium tuberculosis, the Rv1460-Rv1461-Rv1462-Rv1463-csd-Rv1465-Rv1466 operon (suf operon) encodes the primary Fe-S cluster biogenesis system. The first gene in this operon, Rv1460, shares homology with the cyanobacterial SufR, which functions as a transcriptional repressor of the sufBCDS operon. Rv1460's function in M. tuberculosis has however not been determined. In this study, we demonstrate that M. tuberculosis mutants lacking a functional Rv1460 protein are impaired for growth under standard culture conditions. Elevated expression of Rv1460 and Rv1461 was observed in the mutant, implicating Rv1460 in the regulation of the suf operon. Binding of an Fe-S cluster to purified recombinant Rv1460 was confirmed by UV-visible spectroscopy and circular dichroism. Furthermore, three conserved cysteine residues, C203, C216 and C244, proposed to provide ligands for the coordination of an Fe-S cluster, were shown to be required for the function of Rv1460 in M. tuberculosis. Rv1460 therefore seems to be functionally analogous to cyanobacterial SufR.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0200145