Human immune cell engraftment does not alter development of severe acute Rift Valley fever in mice

Rift Valley fever (RVF) in humans is usually mild, but, in a subset of cases, can progress to severe hepatic and neurological disease. Rodent models of RVF generally develop acute severe clinical disease. Here, we inoculated humanized NSG-SGM3 mice with Rift Valley fever virus (RVFV) to investigate...

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Bibliographic Details
Published in:PloS one 2018-07, Vol.13 (7), p.e0201104-e0201104
Main Authors: Spengler, Jessica R, McElroy, Anita K, Harmon, Jessica R, Coleman-McCray, JoAnn D, Welch, Stephen R, Keck, James G, Nichol, Stuart T, Spiropoulou, Christina F
Format: Article
Language:English
Subjects:
Animal models
Biology and life sciences
Coccidioidomycosis
Development and progression
Disease control
Disease prevention
Euthanasia
Fever
Hepatitis
Human behavior
Illnesses
Immune system
Infections
Infectious diseases
Inoculation
Laboratory animals
Liver diseases
Medical laboratories
Medicine and health sciences
Mice
Pathology
Pediatrics
Research and Analysis Methods
Ribonucleic acid
Rift Valley fever
RNA
Rodents
Vector-borne diseases
Viral diseases
Virology
Viruses
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Summary:Rift Valley fever (RVF) in humans is usually mild, but, in a subset of cases, can progress to severe hepatic and neurological disease. Rodent models of RVF generally develop acute severe clinical disease. Here, we inoculated humanized NSG-SGM3 mice with Rift Valley fever virus (RVFV) to investigate whether the presence of human immune cells in mice would alter the progression of RVFV infection to more closely model human disease. Despite increased human cytokine expression, including responses mirroring those seen in human disease, and decreased hepatic viral RNA levels at terminal euthanasia, both high- and low-dose RVFV inoculation resulted in lethal disease in all mice with comparable time-to-death as unengrafted mice.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0201104