Expression of E-prostanoid receptors in nasal polyp tissues of smoking and nonsmoking patients with chronic rhinosinusitis

• Published in: PloS one 2018-07, Vol.13 (7), p.e0200989-e0200989
• Main Authors: Xie, Li, Liu, Ai-Guo, Peng, Li-Yan, Wang, Su-Jie, Zhang, Yin-Ping, Wang, Xian-Song
• Format: Article
• Language: English
• Subjects: Adult; Asthma; Biology and Life Sciences; Biopsy; Chronic Disease; Cigarette smoke; Cigarettes; Endoscopy; Enzyme-linked immunosorbent assay; Exposure; Female; Gene expression; Gene Expression Regulation; Genetic aspects; Health aspects; Humans; Inflammation; Interleukin 8; Male; Medicine and Health Sciences; Middle Aged; Nasal polyps; Nasal Polyps - metabolism; Otolaryngology; Pathogenesis; Patients; Physiological aspects; Polymerase chain reaction; Polyps; Prostaglandin E2; Prostaglandin receptors; Prostanoids; Proteins; Receptors; Receptors, Prostaglandin E - genetics; Receptors, Prostaglandin E - metabolism; Research and Analysis Methods; Rhinitis; Rhinosinusitis; Ribonucleic acid; Risk factors; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sinuses; Sinusitis; Sinusitis - genetics; Sinusitis - metabolism; Smoke; Smoking; Smoking - metabolism; Smooth muscle; Surgery; Tissues; Tomography; Tumor necrosis factor-α; Young Adult
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0200989

Different inflammatory reactions have been observed in the polyp tissues of nonsmokers and smokers with chronic rhinosinusitis (CRS). E-prostanoid (EP) receptors play a role in the inflammatory processes. Cigarette smoke (CS) exposure regulates EP-receptor expression levels promoting inflammatory mediator release from various inflammatory cells. In this study, we characterize the EP-receptor expression profiles in the polyps of nonsmoking and smoking CRS patients to explore the possible role of CS in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). Polyp biopsies were obtained from 28 non-smoking and 21 smoking CRSwNP patients. Histopathological characteristics were observed under a light microscope. The prostaglandin E2 (PGE2), TNF-α, and IL-8 contents in polyp tissues were detected using enzyme-linked immunosorbent assay. Immunostaining was used to locate EP receptors in polyps. Messenger RNA and protein expression of EP receptors were examined using quantitative real-time polymerase chain reaction and Western blot, respectively. More severe inflammatory reactions occurred in polyp tissues of smoking CRSwNP patients. The PGE2, TNF-α, and IL-8 in tissue homogenate levels were significantly higher in smoking CRSwNP patients than those in nonsmoking CRSwNP patients. Moreover, the distribution of each EP receptor subtype was similar in both groups. Compared with the EP-receptor expression in nonsmokers, messenger RNA and protein of EP2 and EP4 receptor were significantly down-expressed in smoking patients, but EP1 and EP3 receptors did not show significant differences. CS exposure downregulates the expression levels of EP2 and EP4 receptors and stimulates the production of PGE2 and the proinflammatory cytokine IL-8 and TNF-α in polyp tissues of CRS patients. The down-expressed EP2 and EP4 receptors might be associated with severe inflammatory reactions in smoking CRSwNP patients.