Image and motor behavior for monitoring tumor growth in C6 glioma model

• Published in: PloS one 2018-07, Vol.13 (7), p.e0201453
• Main Authors: Souza, Taylla Klei Felix, Nucci, Mariana Penteado, Mamani, Javier Bustamante, da Silva, Helio Rodrigues, Fantacini, Daianne Maciely Carvalho, de Souza, Lucas Eduardo Botelho, Picanço-Castro, Virginia, Covas, Dimas Tadeu, Vidoto, Edson Luis, Tannús, Alberto, Gamarra, Lionel Fernel
• Format: Article
• Language: English
• Subjects: Animals; Biology and Life Sciences; Bioluminescence; Brain - physiopathology; Brain cancer; Brain Neoplasms - diagnostic imaging; Brain Neoplasms - physiopathology; Brain research; Care and treatment; Cell culture; Cell Line, Tumor; Cellular signal transduction; Change detection; Correlation; Correlation analysis; Correlation coefficient; Correlation coefficients; Development and progression; Diagnosis; Gait; Gait Analysis; Gene expression; Genetic aspects; Glioma; Glioma - diagnostic imaging; Glioma - physiopathology; Gliomas; Locomotion; Luminescent Measurements - methods; Magnetic resonance; Magnetic resonance imaging; Magnetic Resonance Imaging - methods; Male; Medicine and Health Sciences; Monitoring; Neuroimaging; NMR; Nuclear magnetic resonance; Photons; Rats; Rats, Wistar; Research and Analysis Methods; Rodents; Sensitivity analysis; Tumors
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0201453

The primary objective of this study is to monitor tumor growth by using image techniques and behavioral testing through general and specific motor activities (spontaneous movements and gait). Our sample includes male Wistar rats, 2 months old and weighing 250-300 g, that is categorized into three groups: control, sham, and experimental. The experimental group was anesthetized; the C6 cells with luciferase expression that were suspended in a culture medium were implanted into the right frontoparietal cortex of the rats. The sham group received implant only with culture medium without cells. Images and behavioral tests were evaluated at base time and at 7, 14, 21, and 28 days after induced tumor growth analysis. The tumor volume measured by magnetic resonance imaging (MRI) and quantitative bioluminescence imaging (BLI) signal showed a correlation coefficient of r = 0.96. The MRI showed that the mean tumor volume increased by approximately 10, 26, and 49 times according to a comparison of tumor volume on the seventh day with 14, 21, and 28 days, respectively. The quantification of the BLI signal was (4.12 ± 2.01) x 10(8), (8.33 ± 3.12) x 10(8), (28.43 ± 6.32) x 10(8), and (63.02 ± 10.53) x 10(8) photons/s at the seventh, fourteenth, twenty-first, and twenty-eighth day, respectively. After 14 days of tumor induction, both behavioral tests showed significant differences between tumor and sham or control groups. Our study showed a high correlation between MRI and BLI for tumor growth monitoring with complement aspects analysis in tumor volume. In addition, functional behavioral analysis displayed sensitivity to monitor tumor growth, as well as to detect early significant changes between groups, primarily in the tumor group. The results of gait analysis were more sensitive than general motor analysis.