MiR-221 negatively regulates innate anti-viral response

The innate immune system plays a critical role in the initial antiviral response. However, the timing and duration of these responses must be tightly regulated during infection to ensure appropriate immune cell activation and anti-viral defenses. Here we demonstrate that during antiviral response, a...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2018-08, Vol.13 (8), p.e0200385-e0200385
Main Authors: Du, Hongqiang, Cui, Shuang, Li, Yunfei, Yang, Guang, Wang, Peiyan, Fikrig, Erol, You, Fuping
Format: Article
Language:English
Subjects:
Analysis
Antiviral agents
Antiviral drugs
Autoimmune diseases
Biology
Biology and life sciences
Cell activation
Cloning
Gene expression
Health aspects
Health care
Immune system
Immunology
Infection
Infections
Infectious diseases
Innate immunity
Kinases
Ligands
Medicine and Health Sciences
MicroRNA
MicroRNAs
miRNA
Neurosciences
Pathogenesis
Plasmids
Preventive medicine
Proteins
Research and analysis methods
Ribonucleic acid
RNA
RNA sequencing
RNA viruses
Transcription factors
Viral infections
Virus diseases
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The innate immune system plays a critical role in the initial antiviral response. However, the timing and duration of these responses must be tightly regulated during infection to ensure appropriate immune cell activation and anti-viral defenses. Here we demonstrate that during antiviral response, a negative regulator miR-221 was also induced in an ELF4-dependent manner. We further show that ELF4 promotes miR-221 expression through direct binding to its promoter. Overexpression and knockdown assay show that miR-221 can negatively regulate IFNβ production in time of virus infection. RNA-seq analysis of miR-221 overexpressed cells revealed multiple candidate targets. Taken together, our study identified a novel negative microRNA regulator of innate antiviral response, which is dependent on ELF4.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0200385