Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping

• Published in: PloS one 2018-08, Vol.13 (8), p.e0201981-e0201981
• Main Authors: Perea Razo, Claudia Angélica, Rodríguez Hernández, Elba, Ponce, Sergio Iván Román, Milián Suazo, Feliciano, Robbe-Austerman, Suelee, Stuber, Tod, Cantó Alarcón, Germinal Jorge
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Bacterial Typing Techniques; Beef cattle; Biology and Life Sciences; Bovine tuberculosis; Cattle; Clustering; Developing countries; DNA sequencing; Epidemiology; Evolution, Molecular; Gene sequencing; Genetic analysis; Genetic diversity; Genetic Variation; Genome, Bacterial; Genomes; Genotyping; Health risks; Internet; Laboratories; LDCs; Medicine and Health Sciences; Mexico - epidemiology; Molecular Epidemiology; Mycobacterium bovis - genetics; Pathogens; People and places; Phylogeny; Polymorphism, Single Nucleotide; Research and Analysis Methods; Resolution; Spoligotyping; Studies; Tuberculosis; Tuberculosis, Bovine - epidemiology; Tuberculosis, Bovine - microbiology; Whole Genome Sequencing
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0201981

Mycobacterium bovis infection in cattle persists in Mexico, posing a threat to human health. Control of bovine tuberculosis, through the National Program Against Bovine Tuberculosis, has led to the decrease of disease prevalence in most of the country, except for high dairy production regions. Genotyping of M. bovis has been performed mainly by spoligotyping and variable number tandem repeats (VNTR), but higher resolution power can be useful for a finer definition of the spread of the disease. Whole genome sequencing and spoligotyping was performed for a set of 322 M. bovis isolates from different sources in Mexico: Baja California, Coahuila, Estado de Mexico, Guanajuato, Hidalgo, Jalisco, Queretaro and Veracruz, from dairy and beef cattle, as well as humans. Twelve main genetic clades were obtained through WGS and genetic diversity analysis. A clear differentiation of the Baja California isolates was seen as they clustered together exclusively. However, isolates from the central states showed no specific clustering whatsoever. Although WGS proves to have higher resolving power than spoligotyping, and since there was concordance between WGS and spoligotyping results, we consider that the latter is still an efficient and practical method for monitoring bovine tuberculosis in developing countries, where resources for higher technology are scarce.