Interactions of free-living amoebae with rice bacterial pathogens Xanthomonas oryzae pathovars oryzae and oryzicola

Free-living amoebae (FLA) are voracious feeders, consuming bacteria and other microbes during colonization of the phytobiome. FLA are also known to secrete bacteriocidal or bacteriostatic compounds into their growth environment. Here, we explore the impacts of co-cultivation of five FLA species, inc...

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Bibliographic Details
Published in:PloS one 2018-08, Vol.13 (8), p.e0202941-e0202941
Main Authors: Long, John J, Jahn, Courtney E, Sánchez-Hidalgo, Andrea, Wheat, William, Jackson, Mary, Gonzalez-Juarrero, Mercedes, Leach, Jan E
Format: Article
Language:English
Subjects:
Amoeba
Amoeba - physiology
Amoebidae
Analysis
Bacteria
Biofilms
Biology and Life Sciences
Colonization
Crop diseases
Cultivation
Feeders
Immunology
Leprosy
Microorganisms
Oryza - microbiology
Pathogenic microorganisms
Pathogens
Pathology
Plant bacterial diseases
Predation
Research and Analysis Methods
Rice
Trophozoites - physiology
Xanthomonas - cytology
Xanthomonas - physiology
Xanthomonas oryzae
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Summary:Free-living amoebae (FLA) are voracious feeders, consuming bacteria and other microbes during colonization of the phytobiome. FLA are also known to secrete bacteriocidal or bacteriostatic compounds into their growth environment. Here, we explore the impacts of co-cultivation of five FLA species, including Acanthamoeba castellanii, A. lenticulata, A. polyphaga, Dictyostelium discoideum and Vermamoeba vermiformis, on survival of two devastating bacterial pathogens of rice, Xanthomonas oryzae pathovars (pv.) oryzae and oryzicola. In co-cultivation assays, the five FLA species were either bacteriostatic or bactericidal to X. oryzae pv. oryzae and X. oryzae pv. oryzicola. Despite these effects, bacteria were rarely detected inside amoebal cells. Furthermore, amoebae did not disrupt X. oryzae biofilms. The bactericidal effects persisted when bacteria were added to a cell-free supernatant from amoebal cultures, suggesting some amoebae produce an extracellular bactericidal compound. This work establishes novel, basal dynamics between important plant pathogenic bacteria and diverse amoebae, and lays the framework for future mechanistic studies.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0202941