Quantitative lipidomic analysis of mouse lung during postnatal development by electrospray ionization tandem mass spectrometry

• Published in: PloS one 2018-09, Vol.13 (9), p.e0203464-e0203464
• Main Authors: Karnati, Srikanth, Garikapati, Vannuruswamy, Liebisch, Gerhard, Van Veldhoven, Paul P, Spengler, Bernhard, Schmitz, Gerd, Baumgart-Vogt, Eveline
• Format: Article
• Language: English
• Subjects: Age Factors; Alveoli; Analysis; Analytical chemistry; Anatomy & physiology; Animals; Animals, Newborn; Atomic properties; Biology; Biology and Life Sciences; Biosynthesis; Carbon; Cholesterol; Cholesterol - analysis; Cholesterol - metabolism; Cholesterol Esters - analysis; Cholesterol Esters - metabolism; Chronic obstructive pulmonary disease; Composition; Disease; Esters; Fatty Acids - analysis; Fatty Acids - metabolism; Female; Health aspects; Ionization; Laboratories; Lipids; Lipids - analysis; Lung; Lung - chemistry; Lung - growth & development; Lung diseases; Lungs; Male; Mass spectrometry; Mass spectroscopy; Mice, Inbred C57BL; Palmitic acid; Phospholipids - analysis; Phospholipids - metabolism; Physical Sciences; Physiological aspects; Physiology; Respiratory diseases; Respiratory distress syndrome; Scientific imaging; Species composition; Spectrometry, Mass, Electrospray Ionization - methods; Sphingolipids - analysis; Sphingolipids - metabolism; Studies; Surface tension; Surfactants; Tandem Mass Spectrometry - methods
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0203464

Lipids play very important roles in lung biology, mainly reducing the alveolar surface tension at the air-liquid interface thereby preventing end-expiratory collapse of the alveoli. In the present study we performed an extensive quantitative lipidomic analysis of mouse lung to provide the i) total lipid quantity, ii) distribution pattern of the major lipid classes, iii) composition of individual lipid species and iv) glycerophospholipid distribution pattern according to carbon chain length (total number of carbon atoms) and degree of unsaturation (total number of double bonds). We analysed and quantified 160 glycerophospholipid species, 24 sphingolipid species, 18 cholesteryl esters and cholesterol from lungs of a) newborn (P1), b) 15-day-old (P15) and c) 12-week-old adult mice (P84) to understand the changes occurring during postnatal pulmonary development. Our results revealed an increase in total lipid quantity, correlation of lipid class distribution in lung tissue and significant changes in the individual lipid species composition during postnatal lung development. Interestingly, we observed significant stage-specific alterations during this process. Especially, P1 lungs showed high content of monounsaturated lipid species; P15 lungs exhibited myristic and palmitic acid containing lipid species, whereas adult lungs were enriched with polyunsaturated lipid species. Taken together, our study provides an extensive quantitative lipidome of the postnatal mouse lung development, which may serve as a reference for a better understanding of lipid alterations and their functions in lung development and respiratory diseases associated with lipids.