Comparative transcriptome analysis of Eriocheir japonica sinensis response to environmental salinity

• Published in: PloS one 2018-09, Vol.13 (9), p.e0203280-e0203280
• Main Authors: Zhang, Daizhen, Liu, Jun, Qi, Tingting, Ge, Baoming, Liu, Qiuning, Jiang, Senhao, Zhang, Huabin, Wang, Zhengfei, Ding, Ge, Tang, Boping
• Format: Article
• Language: English
• Subjects: 14-3-3 protein; Acclimatization - genetics; Adaptation; Analysis; Animals; Annotations; Antioxidants - metabolism; Aquatic habitats; Arsenite; Biological evolution; Biology and Life Sciences; Brachyura - genetics; Brachyura - physiology; Calcium; Calcium ions; Cellular signal transduction; Chinese mitten crab; Citric Acid Cycle - genetics; Copper; Crabs; Crustaceans; Earth Sciences; Ecology and Environmental Sciences; Environmental protection; Enzymes; Eriocheir japonica; Fresh Water; Freshwater environments; Gene Expression; Gene Expression Profiling; Genes; Genomes; H+-transporting ATPase; Hemolymph - metabolism; Immune system; Ion Transport - genetics; Laboratories; Life history; Male; Marine environment; Metabolic Networks and Pathways - genetics; Metabolism; Molecular Sequence Annotation; Na+/K+-exchanging ATPase; Osmoregulation; Osmoregulation - genetics; Oxidative Phosphorylation; Physiology; Salinity; Salinity effects; Seawater; Sequence Analysis, RNA; Shellfish; Signal Transduction - genetics; Transduction; Transport; Wetlands
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0203280

Chinese mitten crabs (Eriocheir japonica sinensis) are catadromous, spending most of their lives in fresh water, but moving to a mixed salt-fresh water environment for reproduction. The characteristics of this life history might imply a rapidly evolutionary transition model for adaptation to marine from freshwater habitats. In this study, transcriptome-wide identification and differential expression on Chinese mitten crab groups were analysed. Results showed: clean reads that were obtained totalled 93,833,096 (47,440,998 in Group EF, the reference, and 46,392,098 in Group ES, the experimental) and 14.08G (7.12G in Group EF 6.96G in Group ES); there were 11,667 unigenes (15.29%) annotated, and they were located to 230 known KEGG pathways in five major categories; in differential expression analysis, most of the top 20 up-regulated pathways were connected to the immune system, disease, and signal transduction, while most of the top 20 down-regulated pathways were related to the metabolism system; meanwhile, 8 representative osmoregulation-related genes (14-3-3 epsilon, Cu2+ transport ATPase, Na+/K+ ATPase, Ca2+ transporting ATPase, V-ATPase subunit A, Putative arsenite-translocating ATPase, and Cation transport ATPase, Na+/K+ symporter) showed up-regulation, and 1 osmoregulation-related gene (V-ATPase subunit H) showed down-regulation. V-ATPase subunit H was very sensitive to the transition of habitats. These results were consistent with the tests of qRT-PCR. The present study has provided a foundation to further understand the molecular mechanism in response to salinity changing in water.