Transgenic Metarhizium pingshaense synergistically ameliorates pyrethroid-resistance in wild-caught, malaria-vector mosquitoes

Transgenic Metarhizium pingshaense expressing the spider neurotoxin Hybrid (Met-Hybrid) kill mosquitoes faster and at lower spore doses than wild-type strains. In this study, we demonstrate that this approach dovetails with the cornerstone of current malaria control: pyrethroid-insecticides, which a...

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Published in:PloS one 2018-09, Vol.13 (9), p.e0203529
Main Authors: Bilgo, Etienne, Lovett, Brian, Bayili, Koama, Millogo, Abel Souro, Saré, Issiaka, Dabiré, Roch K, Sanon, Antoine, St Leger, Raymond J, Diabate, Abdoulaye
Format: Article
Language:English
Subjects:
Animals
Anopheles - drug effects
Anopheles - microbiology
Aquatic insects
Ascomycota
Bioassays
Biological control
Biological Control Agents - pharmacology
Biological effects
Biology and Life Sciences
Biopesticides
Burkina Faso
Care and treatment
Control
Copyright
Culicidae
Drug Synergism
Environmental aspects
Female
Fluorescence
Fungi
Genetic engineering
Health aspects
Impact resistance
Infections
Insecticide resistance
Insecticide Resistance - drug effects
Insecticide-Treated Bednets
Insecticides
Insecticides - pharmacology
Laboratories
Malaria
Medicine and Health Sciences
Metarhizium
Metarhizium - genetics
Metarhizium - growth & development
Mosquito Control - methods
Mosquito Vectors - drug effects
Mosquito Vectors - microbiology
Mosquitoes
Neurotoxins
Neurotoxins - genetics
Parasites
Permethrin
Permethrin - pharmacology
Pesticide resistance
Pyrethrins - pharmacology
Pyrethroids
Tubes
Vector-borne diseases
Vectors
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cited_by cdi_FETCH-LOGICAL-c692t-1b832e8ce48b2b47ec78c76ef373202eed30899ba25858a094cf2e2bc65824c73
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container_issue 9
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creator Bilgo, Etienne
Lovett, Brian
Bayili, Koama
Millogo, Abel Souro
Saré, Issiaka
Dabiré, Roch K
Sanon, Antoine
St Leger, Raymond J
Diabate, Abdoulaye
description Transgenic Metarhizium pingshaense expressing the spider neurotoxin Hybrid (Met-Hybrid) kill mosquitoes faster and at lower spore doses than wild-type strains. In this study, we demonstrate that this approach dovetails with the cornerstone of current malaria control: pyrethroid-insecticides, which are the cornerstone of current malaria control. We used World Health Organization (WHO) tubes, to compare the impact on insecticide resistance of Met-Hybrid with red fluorescent M. pingshaense (Met-RFP), used as a proxy for the wild-type fungus. Insecticides killed less than 20% of Anopheles coluzzii and Anopheles gambiae s.s. mosquitoes collected in a malaria endemic region of Burkina Faso where pyrethroid use is common. Seven days post-infection, mortality for insecticide-sensitive and resistant mosquitoes averaged 94% with Met-Hybrid and 64% with Met-RFP, with LT80 values of 5.32±0.199 days and 7.76±0.183 days, respectively. Eighty nine percent of insecticide-resistant mosquitoes exposed to permethrin five days post-infection with Met-Hybrid died within 24 hours: only 22% died from Met-Hybrid alone over this 24-hour period. Compared to Met-RFP, Met-Hybrid also significantly reduced flight capacity of mosquitoes 3 to 5 days post-infection. Based on WHOPES phase I laboratory susceptibility bioassays, transgenic Met-Hybrid provides effective biological control for adult African malaria vectors that may be used to synergistically manage insecticide resistance with current methods.
doi_str_mv 10.1371/journal.pone.0203529
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Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bilgo, Etienne</au><au>Lovett, Brian</au><au>Bayili, Koama</au><au>Millogo, Abel Souro</au><au>Saré, Issiaka</au><au>Dabiré, Roch K</au><au>Sanon, Antoine</au><au>St Leger, Raymond J</au><au>Diabate, Abdoulaye</au><au>Favia, Guido</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transgenic Metarhizium pingshaense synergistically ameliorates pyrethroid-resistance in wild-caught, malaria-vector mosquitoes</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2018-09-07</date><risdate>2018</risdate><volume>13</volume><issue>9</issue><spage>e0203529</spage><pages>e0203529-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Transgenic Metarhizium pingshaense expressing the spider neurotoxin Hybrid (Met-Hybrid) kill mosquitoes faster and at lower spore doses than wild-type strains. In this study, we demonstrate that this approach dovetails with the cornerstone of current malaria control: pyrethroid-insecticides, which are the cornerstone of current malaria control. We used World Health Organization (WHO) tubes, to compare the impact on insecticide resistance of Met-Hybrid with red fluorescent M. pingshaense (Met-RFP), used as a proxy for the wild-type fungus. Insecticides killed less than 20% of Anopheles coluzzii and Anopheles gambiae s.s. mosquitoes collected in a malaria endemic region of Burkina Faso where pyrethroid use is common. Seven days post-infection, mortality for insecticide-sensitive and resistant mosquitoes averaged 94% with Met-Hybrid and 64% with Met-RFP, with LT80 values of 5.32±0.199 days and 7.76±0.183 days, respectively. Eighty nine percent of insecticide-resistant mosquitoes exposed to permethrin five days post-infection with Met-Hybrid died within 24 hours: only 22% died from Met-Hybrid alone over this 24-hour period. Compared to Met-RFP, Met-Hybrid also significantly reduced flight capacity of mosquitoes 3 to 5 days post-infection. Based on WHOPES phase I laboratory susceptibility bioassays, transgenic Met-Hybrid provides effective biological control for adult African malaria vectors that may be used to synergistically manage insecticide resistance with current methods.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30192847</pmid><doi>10.1371/journal.pone.0203529</doi><tpages>e0203529</tpages><orcidid>https://orcid.org/0000-0003-2350-9339</orcidid><oa>free_for_read</oa></addata></record>
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identifier ISSN: 1932-6203
ispartof PloS one, 2018-09, Vol.13 (9), p.e0203529
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_2100863634
source Publicly Available Content Database; PubMed Central
subjects Animals
Anopheles - drug effects
Anopheles - microbiology
Aquatic insects
Ascomycota
Bioassays
Biological control
Biological Control Agents - pharmacology
Biological effects
Biology and Life Sciences
Biopesticides
Burkina Faso
Care and treatment
Control
Copyright
Culicidae
Drug Synergism
Environmental aspects
Female
Fluorescence
Fungi
Genetic engineering
Health aspects
Impact resistance
Infections
Insecticide resistance
Insecticide Resistance - drug effects
Insecticide-Treated Bednets
Insecticides
Insecticides - pharmacology
Laboratories
Malaria
Medicine and Health Sciences
Metarhizium
Metarhizium - genetics
Metarhizium - growth & development
Mosquito Control - methods
Mosquito Vectors - drug effects
Mosquito Vectors - microbiology
Mosquitoes
Neurotoxins
Neurotoxins - genetics
Parasites
Permethrin
Permethrin - pharmacology
Pesticide resistance
Pyrethrins - pharmacology
Pyrethroids
Tubes
Vector-borne diseases
Vectors
title Transgenic Metarhizium pingshaense synergistically ameliorates pyrethroid-resistance in wild-caught, malaria-vector mosquitoes
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