Factors affecting soil microbial biomass and functional diversity with the application of organic amendments in three contrasting cropland soils during a field experiment

• Published in: PloS one 2018-09, Vol.13 (9), p.e0203812-e0203812
• Main Authors: Li, Ling, Xu, Minggang, Eyakub Ali, Mohammad, Zhang, Wenju, Duan, Yinghua, Li, Dongchu
• Format: Article
• Language: English
• Subjects: Agricultural land; Animal wastes; Biology and Life Sciences; Biomass; Carbon; Carbon/nitrogen ratio; Cattle manure; Climate change; Corn; Corn straw; Crop diversification; Crop residues; Diversity indices; Ecology and Environmental Sciences; Ecosystems; Energy crops; Engineering; Forest soils; Laboratories; Lignin; Manures; Mathematical analysis; Metabolism; Microorganisms; Nitrogen; Organic materials; Organic soils; Physical Sciences; Pig manure; Quality; Research and Analysis Methods; Soil carbon; Soil contamination; Soil fertility; Soil microbiology; Soil microorganisms; Soil properties; Soil types; Straw; Substrates; Wheat; Wheat straw
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0203812

The effects of soil type and organic material quality on the microbial biomass and functional diversity of cropland soils were studied in a transplant experiment in the same climate during a 1-year field experiment. Six organic materials (WS: wheat straw, CS: corn straw, WR: wheat root, CR: corn root, PM: pig manure, CM: cattle manure), and three contrasting soils (Ferralic Cambisol, Calcaric Cambisol and Luvic Phaeozem) were chosen. At two time points (at the end of the 1st and 12th months), soil microbial biomass carbon (C) and nitrogen (N) (MBC and MBN) and Biolog Ecoplate substrate use patterns were determined, and the average well color development and the microbial functional diversity indices (Shannon, Simpson and McIntosh indices) were calculated. Organic material quality explained 29.5-50.9% of the variance in MBC and MBN when compared with the minor role of soil type (1.4-9.3%) at the end of the 1st and 12th months, and C/N ratio and total N of organic material were the main parameters. Soil properties, e.g., organic C and clay content were the predominant influence on microbial functional diversity in particular at the end of the 12th month (61.8-82.8% of the variance explained). The treatments of WS and CS significantly improved the MBC and microbial functional diversity indices over the control in the three soils in both sampling periods (P < 0.05). These results suggest that the application of crop straw is a long-term effective measure to increase microbial biomass, and can further induce the changes of soil properties to regulate soil microbial community.