Induction of obesity impairs reverse cholesterol transport in ob/ob mice

• Published in: PloS one 2018-09, Vol.13 (9), p.e0202102-e0202102
• Main Authors: Duong, MyNgan, Uno, Kiyoko, Nankivell, Victoria, Bursill, Christina, Nicholls, Stephen J
• Format: Article
• Language: English
• Subjects: Adipocytes; Adipocytes - metabolism; Adipocytes - pathology; Adipose tissue; Adipose Tissue - metabolism; Adipose Tissue - pathology; Animal models; Animal tissues; Animals; Apolipoprotein A-I - metabolism; Atherosclerosis; ATP Binding Cassette Transporter, Subfamily G, Member 1 - metabolism; Bile; Biological Transport, Active; Biology; Biology and Life Sciences; Body fat; Cardiovascular diseases; Cholesterol; Cholesterol - metabolism; Control methods; Cytokines; Drug dosages; Efflux; Health risks; Heart; Hepatocytes; Hepatocytes - metabolism; Hepatocytes - pathology; High density lipoprotein; Laboratory animals; Lipids; Lipoproteins, HDL - metabolism; Liver; Liver - metabolism; Liver - pathology; Low density lipoprotein; Macrophages; Macrophages - metabolism; Macrophages - pathology; Medical research; Medicine and Health Sciences; Metabolism; Mice; Mice, Obese; Obesity; Obesity - metabolism; Obesity - pathology; Proteins; Research and Analysis Methods; Risk analysis; Risk factors; Scavenger Receptors, Class B - metabolism; Transport
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0202102

Obesity is an independent risk factor for cardiovascular disease. Reverse cholesterol transport (RCT) is an important cardioprotective mechanism. This study aimed to investigate RCT changes in a murine model of obesity. Ob/ob and control mice were injected with [3H]-cholesterol-labelled macrophages and cholesterol accumulation quantified after 48 h. Ex vivo, cholesterol efflux and uptake were determined in hepatic and adipose tissues. Ob/ob mice had more labelled cholesterol in their plasma (86%, p