An integrated analysis of mRNA and sRNA transcriptional profiles in tomato root: Insights on tomato wilt disease

Tomato wilt disease caused by Fusarium oxysporum f. sp. lycopersici (FOL) is a worldwide destructive disease of tomato. As exploring gene expression and function approaches constitute an initial point for investigating pathogen-host interaction, we performed RNA-seq and sRNA-seq analysis to investig...

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Bibliographic Details
Published in:PloS one 2018-11, Vol.13 (11), p.e0206765-e0206765
Main Authors: Zhao, Min, Ji, Hui-Min, Gao, Ying, Cao, Xin-Xin, Mao, Hui-Ying, Ouyang, Shou-Qiang, Liu, Peng
Format: Article
Language:English
Subjects:
Binding sites
Biology and life sciences
Catalysis
Catalytic activity
Cultivars
Fusarium oxysporum
Gene expression
Genes
Genetic aspects
Genetic transcription
Genomics
Horticulture
Host-bacteria relationships
Infections
Inoculation
Kinases
Messenger RNA
MicroRNAs
miRNA
Physiological aspects
Plant resistance
Polymerase chain reaction
Proteins
Research and analysis methods
Ribonucleic acid
RNA
Tomatoes
Transcription
Wilt
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Summary:Tomato wilt disease caused by Fusarium oxysporum f. sp. lycopersici (FOL) is a worldwide destructive disease of tomato. As exploring gene expression and function approaches constitute an initial point for investigating pathogen-host interaction, we performed RNA-seq and sRNA-seq analysis to investigate the transcriptome of tomato root under FOL infection. Differentially expressed (DE) protein-coding gene and miRNA gene profiles upon inoculation with FOL were presented at twenty-four hours post-inoculation in four treatments. A total of more than 182.6 million and 132.2 million high quality clean reads were obtained by RNA-seq and sRNA-seq, respectively. A large overlap was found in DE mRNAs between susceptible cultivar Moneymaker and resistant cultivar Motelle. Gene Ontology terms were mainly classified into catalytic activity, metabolic process and binding. Combining with qRT-PCR and Northern blot, we validated the transcriptional profile of five genes and five miRNAs conferred to FOL infection. Our work allowed comprehensive understanding of different transcriptional reaction of genes/miRNAs between the susceptible and resistant cultivars tomato to the FOL challenge, which could offer us with a future direction to generate models of mediated resistance responses.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0206765