Longitudinal analysis of developmental changes in electroencephalography patterns and sleep-wake states of the neonatal mouse

• Published in: PloS one 2018-11, Vol.13 (11), p.e0207031-e0207031
• Main Authors: Rensing, Nicholas, Moy, Brianna, Friedman, Joseph L, Galindo, Rafael, Wong, Michael
• Format: Article
• Language: English
• Subjects: Age; Animal models; Animals; Animals, Newborn - growth & development; Biology and Life Sciences; Brain; Brain - pathology; Brain injury; Brain research; Control data (computers); Convulsions & seizures; EEG; Electrodes; Electrodes, Implanted; Electroencephalography; Electromyography; Emergence; Fast Fourier transformations; Female; Fourier transforms; Head injuries; Hypoxia; Injury analysis; Laboratory animals; Longitudinal Studies; Male; Medicine; Medicine and Health Sciences; Mice; Neonates; Nervous system; Neurological disorders; Neurology; Newborn babies; NREM sleep; Recording; REM sleep; Research and Analysis Methods; Rodents; Skull; Sleep; Sleep (NREM); Sleep (REM); Sleep - physiology; Sleep and wakefulness; Sleep, REM - physiology; Stainless steel; Vigilance; Vigilance (Psychology); Wakefulness - physiology; Wire
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0207031

The neonatal brain undergoes rapid maturational changes that facilitate the normal development of the nervous system and also affect the pathological response to brain injury. Electroencephalography (EEG) and analysis of sleep-wake vigilance states provide important insights into the function of the normal and diseased immature brain. While developmental changes in EEG and vigilance states are well-described in people, less is known about the normal maturational properties of rodent EEG, including the emergence and evolution of sleep-awake vigilance states. In particular, a number of developmental EEG studies have been performed in rats, but there is limited comparable research in neonatal mice, especially as it pertains to longitudinal EEG studies performed within the same mouse. In this study, we have attempted to provide a relatively comprehensive assessment of developmental changes in EEG background activity and vigilance states in wild-type mice from postnatal days 9-21. A novel EEG and EMG method allowed serial recording from the same mouse pups. EEG continuity and power and vigilance states were analyzed by quantitative assessment and fast Fourier transforms. During this developmental period, we demonstrate the timing of maturational changes in EEG background continuity, frequencies, and power and the emergence of identifiable wake, NREM, and REM sleep states. These results should serve as important control data for physiological studies of mouse models of normal brain development and neurological disease.