Filter paper-based spin column method for cost-efficient DNA or RNA purification

We describe herein a method of recharging used commercial spin columns or assembling homemade spin columns using filter paper as binding material for cost-effective, low throughput nucleic acid purification. The efficiency of filter paper-based spin columns was evaluated for purification of nucleic...

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Bibliographic Details
Published in:PloS one 2018-12, Vol.13 (12), p.e0203011-e0203011
Main Authors: Shi, Rui, Lewis, Ramsey S, Panthee, Dilip R
Format: Article
Language:English
Subjects:
Analysis
Backup software
Binding
Biology and Life Sciences
Buffers
Cellulose
Centrifugation - methods
Crops
Deoxyribonucleic acid
DNA
DNA, Plant - chemistry
DNA, Plant - isolation & purification
Economic aspects
Engineering and Technology
Filter paper
Gels
Horticulture
Laboratories
Methods
Molecular biology
Nicotiana - chemistry
Nucleic acids
Physical Sciences
Plasmids
Purification
Recharging
Research and analysis methods
Ribonucleic acid
RNA
RNA, Plant - chemistry
RNA, Plant - isolation & purification
Soil sciences
Solanum lycopersicum - chemistry
Tobacco
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Summary:We describe herein a method of recharging used commercial spin columns or assembling homemade spin columns using filter paper as binding material for cost-effective, low throughput nucleic acid purification. The efficiency of filter paper-based spin columns was evaluated for purification of nucleic acids from various sources. Following protocols of commercial kits, we found filter paper to be a useful binding material for purification of nucleic acids, including plant genomic DNA, plant total RNA, PCR products, and DNA from agarose gels. However, filter paper has a weak binding affinity to plasmid DNA in tested miniprep protocols. Protocols for the use of filter paper recharged spin columns or homemade spin columns for low throughput purification of plant genomic DNA and total RNA with unused commercial kit buffers or less expensive homemade buffers are presented.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0203011