A role for Gic1 and Gic2 in Cdc42 polarization at elevated temperature

The conserved Rho-family GTPase Cdc42 is a master regulator of polarity establishment in many cell types. Cdc42 becomes activated and concentrated in a region of the cell cortex, and recruits a variety of effector proteins to that site. In turn, many effectors participate in regulation of cytoskelet...

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Bibliographic Details
Published in:PloS one 2018-12, Vol.13 (12), p.e0200863-e0200863
Main Authors: Daniels, Christine N, Zyla, Trevin R, Lew, Daniel J
Format: Article
Language:English
Subjects:
Actin
Actin Cytoskeleton - genetics
Actin Cytoskeleton - metabolism
Adaptor Proteins, Signal Transducing - genetics
Adaptor Proteins, Signal Transducing - metabolism
Baking yeast
Biology and Life Sciences
Cancer
cdc42 GTP-Binding Protein - genetics
cdc42 GTP-Binding Protein - metabolism
Cdc42 protein
Cell cycle
Cell growth
Cell proliferation
Cytoskeleton
Effectors
Feedback
Feedback loops
Guanine
Guanine nucleotide exchange factor
Guanosine triphosphatases
Guanosine triphosphate
High temperature
Hot Temperature
Kinases
Molecular biology
Morphogenesis
Pharmacology
Phosphorylation
Polarity
Polarization
Positive feedback
Proteins
Research and Analysis Methods
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Septin
Septins - genetics
Septins - metabolism
Yeast
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Summary:The conserved Rho-family GTPase Cdc42 is a master regulator of polarity establishment in many cell types. Cdc42 becomes activated and concentrated in a region of the cell cortex, and recruits a variety of effector proteins to that site. In turn, many effectors participate in regulation of cytoskeletal elements in order to remodel the cytoskeleton in a polarized manner. The budding yeast Saccharomyces cerevisiae has served as a tractable model system for studies of cell polarity. In yeast cells, Cdc42 polarization involves a positive feedback loop in which effectors called p21-activated kinases (PAKs) act to recruit a Cdc42-directed guanine nucleotide exchange factor (GEF), generating more GTP-Cdc42 in areas that already have GTP-Cdc42. The GTPase-interacting components (GICs) Gic1 and Gic2 are also Cdc42 effectors, and have been implicated in regulation of the actin and septin cytoskeleton. However, we report that cells lacking GICs are primarily defective in polarizing Cdc42 itself, suggesting that they act upstream as well as downstream of Cdc42 in yeast. Our findings suggest that feedback pathways involving GTPase effectors may be more prevalent than had been appreciated.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0200863