Agroecological coffee management increases arbuscular mycorrhizal fungi diversity

• Published in: PloS one 2019-01, Vol.14 (1), p.e0209093-e0209093
• Main Authors: Prates Júnior, Paulo, Moreira, Bruno Coutinho, da Silva, Marliane de Cássia Soares, Veloso, Tomas Gomes Reis, Stürmer, Sidney Luiz, Fernandes, Raphael Bragança Alves, Mendonça, Eduardo de Sá, Kasuya, Maria Catarina Megumi
• Format: Article
• Language: English
• Subjects: Agricultural management; Agriculture; Agriculture - methods; Agroecology; Agroecosystems; Arbuscular mycorrhizas; Biodiversity; Bioinformatics; Biology and Life Sciences; Coffea - microbiology; Coffee; Coffee production; Communities; Community composition; Community ecology; Composition; Computational Biology; Crop production; Crops; Crops, Agricultural - microbiology; Diversity indices; Ecology and Environmental Sciences; Ecosystem; Ecosystem services; Ecosystems; Flowering; Food; Forests; Fungi; Grain; Harvesting; Management; Management systems; Mycorrhizae - classification; Mycorrhizae - genetics; Mycorrhizae - pathogenicity; Polymerase Chain Reaction; Prevention; Soil erosion; Soil improvement; Soil Microbiology; Soil properties; Soil quality; Spores; Studies; Taxonomy; Taxonomy (Biology); Tropical environments
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0209093

Agroecology aims to maintain ecosystem services by minimizing the impact of agriculture and promoting the use of biological potential. Arbuscular mycorrhizal fungi (AMF) are elements which are key to improving crop productivity and soil quality. It is pertinent to understand how agricultural management in the tropics affects the AMF spatio-temporal community composition, especially in crops of global importance, such as coffee (Coffea arabica L.). Soil and root samples were collected from three localities under three management systems (agroecological, conventional and forest fragment), during the phenological stages of coffee (flowering, grain filling, harvesting). Spores were extracted for morphological identification and molecular community analysis by PCR-DGGE. Dendrograms were prepared and the bands were sequenced and analyzed by bioinformatics. No differences were observed in the richness of morphospecies between management systems, localities and period, but little is known about tropical species. Molecular analysis showed that the agroecological management system was similar to natural forest and with a higher diversity indices than conventional management. Locality and period of sample affect AMF community composition. It is necessary to associate classical taxonomic evaluations with molecular biological techniques because different approaches can lead to different outcomes. This study contributes to the understanding of the impact of agriculture management systems on AMF and provides evidence that agroecology is a management system applicable to sustainable coffee production.