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Small molecule inhibition of IRE1α kinase/RNase has anti-fibrotic effects in the lung

Endoplasmic reticulum stress (ER stress) has been implicated in the pathogenesis of idiopathic pulmonary fibrosis (IPF), a disease of progressive fibrosis and respiratory failure. ER stress activates a signaling pathway called the unfolded protein response (UPR) that either restores homeostasis or p...

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Published in:PloS one 2019-01, Vol.14 (1), p.e0209824
Main Authors: Thamsen, Maike, Ghosh, Rajarshi, Auyeung, Vincent C, Brumwell, Alexis, Chapman, Harold A, Backes, Bradley J, Perara, Gayani, Maly, Dustin J, Sheppard, Dean, Papa, Feroz R
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cited_by cdi_FETCH-LOGICAL-c526t-ca72865fe2ab2ab03a097d5b68546b4d9f2a1d847368a320d8d23a79c7c2e9d63
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creator Thamsen, Maike
Ghosh, Rajarshi
Auyeung, Vincent C
Brumwell, Alexis
Chapman, Harold A
Backes, Bradley J
Perara, Gayani
Maly, Dustin J
Sheppard, Dean
Papa, Feroz R
description Endoplasmic reticulum stress (ER stress) has been implicated in the pathogenesis of idiopathic pulmonary fibrosis (IPF), a disease of progressive fibrosis and respiratory failure. ER stress activates a signaling pathway called the unfolded protein response (UPR) that either restores homeostasis or promotes apoptosis. The bifunctional kinase/RNase IRE1α is a UPR sensor/effector that promotes apoptosis if ER stress remains high and irremediable (i.e., a "terminal" UPR). Using multiple small molecule inhibitors against IRE1α, we show that ER stress-induced apoptosis of murine alveolar epithelial cells can be mitigated in vitro. In vivo, we show that bleomycin exposure to murine lungs causes early ER stress to activate IRE1α and the terminal UPR prior to development of pulmonary fibrosis. Small-molecule IRE1α kinase-inhibiting RNase attenuators (KIRAs) that we developed were used to evaluate the contribution of IRE1α activation to bleomycin-induced pulmonary fibrosis. One such KIRA-KIRA7-provided systemically to mice at the time of bleomycin exposure decreases terminal UPR signaling and prevents lung fibrosis. Administration of KIRA7 14 days after bleomycin exposure even promoted the reversal of established fibrosis. Finally, we show that KIRA8, a nanomolar-potent, monoselective KIRA compound derived from a completely different scaffold than KIRA7, likewise promoted reversal of established fibrosis. These results demonstrate that IRE1α may be a promising target in pulmonary fibrosis and that kinase inhibitors of IRE1α may eventually be developed into efficacious anti-fibrotic drugs.
doi_str_mv 10.1371/journal.pone.0209824
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ER stress activates a signaling pathway called the unfolded protein response (UPR) that either restores homeostasis or promotes apoptosis. The bifunctional kinase/RNase IRE1α is a UPR sensor/effector that promotes apoptosis if ER stress remains high and irremediable (i.e., a "terminal" UPR). Using multiple small molecule inhibitors against IRE1α, we show that ER stress-induced apoptosis of murine alveolar epithelial cells can be mitigated in vitro. In vivo, we show that bleomycin exposure to murine lungs causes early ER stress to activate IRE1α and the terminal UPR prior to development of pulmonary fibrosis. Small-molecule IRE1α kinase-inhibiting RNase attenuators (KIRAs) that we developed were used to evaluate the contribution of IRE1α activation to bleomycin-induced pulmonary fibrosis. One such KIRA-KIRA7-provided systemically to mice at the time of bleomycin exposure decreases terminal UPR signaling and prevents lung fibrosis. 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ER stress activates a signaling pathway called the unfolded protein response (UPR) that either restores homeostasis or promotes apoptosis. The bifunctional kinase/RNase IRE1α is a UPR sensor/effector that promotes apoptosis if ER stress remains high and irremediable (i.e., a "terminal" UPR). Using multiple small molecule inhibitors against IRE1α, we show that ER stress-induced apoptosis of murine alveolar epithelial cells can be mitigated in vitro. In vivo, we show that bleomycin exposure to murine lungs causes early ER stress to activate IRE1α and the terminal UPR prior to development of pulmonary fibrosis. Small-molecule IRE1α kinase-inhibiting RNase attenuators (KIRAs) that we developed were used to evaluate the contribution of IRE1α activation to bleomycin-induced pulmonary fibrosis. One such KIRA-KIRA7-provided systemically to mice at the time of bleomycin exposure decreases terminal UPR signaling and prevents lung fibrosis. Administration of KIRA7 14 days after bleomycin exposure even promoted the reversal of established fibrosis. Finally, we show that KIRA8, a nanomolar-potent, monoselective KIRA compound derived from a completely different scaffold than KIRA7, likewise promoted reversal of established fibrosis. These results demonstrate that IRE1α may be a promising target in pulmonary fibrosis and that kinase inhibitors of IRE1α may eventually be developed into efficacious anti-fibrotic drugs.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30625178</pmid><doi>10.1371/journal.pone.0209824</doi><orcidid>https://orcid.org/0000-0002-6982-4863</orcidid><orcidid>https://orcid.org/0000-0001-6273-1595</orcidid><oa>free_for_read</oa></addata></record>
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subjects Alveolar Epithelial Cells - drug effects
Alveolar Epithelial Cells - metabolism
Alveolar Epithelial Cells - pathology
Alveoli
Animals
Apoptosis
Apoptosis - drug effects
Attenuators
Biochemistry
Biology
Biology and Life Sciences
Bleomycin
Cell Line
Critical care
Diabetes
Disease
Endoplasmic reticulum
Endoplasmic Reticulum Stress - drug effects
Endoribonucleases - antagonists & inhibitors
Epithelial cells
Exposure
Fibrosis
Fibrosis - drug therapy
Fibrosis - metabolism
Fibrosis - pathology
Homeostasis
Inhibitors
Kinases
Lung - drug effects
Lung - metabolism
Lung - pathology
Lung diseases
Lungs
Medicine
Medicine and Health Sciences
Mice
Mutation
Pathogenesis
Protein folding
Protein Kinase Inhibitors - pharmacology
Protein Kinase Inhibitors - therapeutic use
Protein Serine-Threonine Kinases - antagonists & inhibitors
Proteins
Pulmonary fibrosis
Research and Analysis Methods
Respiration
Respiratory failure
Signal transduction
Signaling
Sleep
Stress
Surfactants
Transcription factors
Unfolded Protein Response - drug effects
title Small molecule inhibition of IRE1α kinase/RNase has anti-fibrotic effects in the lung
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