Growth factors expression and ultrastructural morphology after application of low-level laser and natural latex protein on a sciatic nerve crush-type injury

The effects of low-level laser therapy (LLLT) and natural latex protein (F1, Hevea brasiliensis) were evaluated on crush-type injuries (15kg) to the sciatic nerve in the expressions of nerve growth factor (NGF) and vascular endothelium growth factor (VEGF) and ultrastructural morphology to associate...

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Bibliographic Details
Published in:PloS one 2019-01, Vol.14 (1), p.e0210211-e0210211
Main Authors: Dias, Fernando José, Fazan, Valéria Paula Sassoli, Cury, Diego Pulzatto, de Almeida, Sonia Regina Yokomizo, Borie, Eduardo, Fuentes, Ramón, Coutinho-Netto, Joaquim, Watanabe, Ii-Sei
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Biology and Life Sciences
Care and treatment
Cell adhesion & migration
Continuous radiation
Crush Injuries - complications
Crush tests
Data processing
Dentistry
Disease Models, Animal
Endothelium
Gene expression
Growth factors
Health aspects
Hevea - chemistry
Humans
Injuries
Intercellular Signaling Peptides and Proteins - metabolism
Laser therapy
Lasers
Latex
Latex - chemistry
Levels
Low-Level Light Therapy
Male
Medicine and Health Sciences
Microscopy, Electron, Transmission
Morphology
Nerve growth factor
Nerve Regeneration - drug effects
Nerve Regeneration - radiation effects
Nervous system
Peripheral Nerve Injuries - etiology
Peripheral Nerve Injuries - therapy
Plant Proteins - administration & dosage
Plant Proteins - isolation & purification
Proteins
R&D
Rats
Rats, Wistar
Recovery of Function
Research & development
Sciatic nerve
Sciatic Nerve - injuries
Sciatic Nerve - physiology
Sciatic Nerve - ultrastructure
Vascular endothelial growth factor
Vascularization
Wound healing
Wound Healing - drug effects
Wound Healing - radiation effects
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Summary:The effects of low-level laser therapy (LLLT) and natural latex protein (F1, Hevea brasiliensis) were evaluated on crush-type injuries (15kg) to the sciatic nerve in the expressions of nerve growth factor (NGF) and vascular endothelium growth factor (VEGF) and ultrastructural morphology to associate with previous morphometric data using the same protocol of injury and treatment. Thirty-six male rats were allocated into six experimental groups (n = 6): 1-Control; 2-Exposed nerve; 3-Injured nerve; 4-LLLT (15J/cm2, 780nm, 30mW, Continuous Wave) treated injured nerve; 5-F1 (0,1mg) treated injured nerve; and 6-LLLT&F1 treated injured nerve. Four or eight weeks after, sciatic nerve samples were processed for analysis. NGF expression were higher (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0210211