Comparisons of early vascular reactions in biodegradable and durable polymer-based drug-eluting stents in the porcine coronary artery

• Published in: PloS one 2019-01, Vol.14 (1), p.e0209841-e0209841
• Main Authors: Ijichi, Takeshi, Nakazawa, Gaku, Torii, Sho, Nagamatsu, Hirofumi, Yoshikawa, Ayako, Souba, Junko, Isobe, Atsushi, Hagiwara, Hitomi, Ikari, Yuji
• Format: Article
• Language: English
• Subjects: Absorbable Implants; Animals; Arteries; Biodegradability; Biodegradation; Biology and Life Sciences; Blood clots; Cardiology; Cardiovascular Agents - administration & dosage; Cardiovascular disease; Coated Materials, Biocompatible - administration & dosage; Coated Materials, Biocompatible - chemistry; Coating; Coatings; Comparative analysis; Coronary Angiography - methods; Coronary artery; Coronary Artery Disease - surgery; Coronary vessels; Coronary Vessels - surgery; Deposition; Drug delivery; Drug-Eluting Stents; Electron microscopy; Endothelial cells; Endothelial Cells - metabolism; Engineering and Technology; Everolimus - administration & dosage; Everolimus - chemistry; Fibrin; Fibrin - metabolism; Health aspects; Hogs; Implantation; Implants; Inflammation; Medicine; Medicine and Health Sciences; Models, Animal; Percutaneous Coronary Intervention; Physical Sciences; Polymer coatings; Polymers; Polymers - administration & dosage; Polymers - adverse effects; Polymers - chemistry; Prosthesis Design; Rapamycin; Research and Analysis Methods; Risk factors; Sirolimus - administration & dosage; Sirolimus - chemistry; Stents; Studies; Surgical implants; Swine; Swine, Miniature; Thrombosis; Thrombosis - etiology; Thrombosis - metabolism; Tight junctions; Transmission electron microscopes; Transmission electron microscopy; Treatment Outcome
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0209841

Current drug-eluting stents have abluminal polymer coating; however, thrombus formation in these compared with that in uniformly coated stents remains controversial. We evaluated thrombus formation and early endothelialization after using abluminal biodegradable polymer-coated sirolimus- (BP-SES), and everolimus-eluting stents (BP-EES) versus a durable polymer-coated everolimus-eluting stent (DP-EES) in an in vivo setting. BP-SES, BP-EES, and DP-EES (n = 6 each) were implanted in coronary arteries of 12 mini-pigs that were then sacrificed after 7 and 10 days. Stents were stained with hematoxylin and eosin, and a combined Verhoeff and Masson trichrome stain. Areas of fibrin deposition were digitally detected and measured with off-line morphometric software. Stents were investigated for re-endothelialization by transmission electron microscopy. At 7 days, histological analysis revealed the lowest area of fibrin deposition in BP-SES (BP-SES vs. BP-EES vs. DP-EES; 0.10 ± 0.06 mm2 vs. 0.15 ± 0.07 mm2 vs. 0.19 ± 0.06 mm2, p = 0.0004). At 10 days, the area of fibrin deposition was significantly greater in DP-EES (0.13 ± 0.04 mm2 vs. 0.14 ± 0.05 mm2 vs. 0.19 ± 0.08 mm2, p = 0.007). Endothelial cells in BP-SES demonstrated a significantly greater number of tight junctions than those in DP-EES according to by transmission electron microscopy for both days (p