The transcriptome sequencing and functional analysis of eyestalk ganglions in Chinese mitten crab (Eriocheir sinensis) treated with different photoperiods

• Published in: PloS one 2019-01, Vol.14 (1), p.e0210414-e0210414
• Main Authors: Pang, Yang-Yang, Zhang, Cong, Xu, Min-Jie, Huang, Gen-Yong, Cheng, Yong-Xu, Yang, Xiao-Zhen
• Format: Article
• Language: English
• Subjects: Adenosine triphosphatase; Animals; Arthropod Proteins - genetics; Arthropod Proteins - physiology; Biology and Life Sciences; Brachyura - genetics; Brachyura - physiology; Care and treatment; Chinese mitten crab; Crabs; Crustaceans; Darkness; Energy metabolism; Eriocheir sinensis; Fish; Functional analysis; Ganglia - physiology; Ganglion cysts; Gene Expression; Gene Expression Profiling; Gene Ontology; Gene sequencing; Health aspects; Kinases; Medicine and Health Sciences; Metabolic Networks and Pathways; Metabolism; Models, Biological; NADH; NADH peroxidase; Nicotinamide adenine dinucleotide; Peroxidase; Photoperiod; Photoperiods; Photoreceptor Cells, Invertebrate - physiology; Phototransduction; Physical Sciences; Physiological effects; Proteins; Pyruvate dehydrogenase (lipoamide); Pyruvate kinase; Pyruvic acid; Real-Time Polymerase Chain Reaction; Research and analysis methods; Ribonucleic acid; RNA; RNA sequencing; Sequence Analysis, RNA; Social Sciences; Studies; Transcriptome; Vision, Ocular - genetics; Vision, Ocular - physiology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0210414

Photoperiod plays an important role in individual growth, development, and metabolism in crustaceans. The growth and reproduction of crabs are closely related to the photoperiod. However, as of yet, there are still no transcriptomic reports of eyestalk ganglions treated under different photoperiods in the Chinese mitten crab (Eriocheir sinensis), which is a benthonic crab with high commercial value in Asia. In this study, we collected the eyestalk ganglions of crabs that were reared under different photoperiods, including a control group (L: D = 12 h: 12 h, named CC), a constant light group (L: D = 24 h: 0 h, named LL) and a constant darkness group (L: D = 0 h: 24 h, named DD). RNA sequencing was performed on these tissues in order to examine the effects of different photoperiods. The total numbers of clean reads from the CC, LL and DD groups were 48,772,584 bp, 53,943,281 bp and 53,815,178 bp, respectively. After de novo assembly, 161,380 unigenes were obtained and were matched with different databases. The DEGs were significantly enriched in phototransduction and energy metabolism pathways. Results from RT-qPCR showed that TRP channel protein (TRP) in the phototransduction pathway had a significantly higher level of expression in LL and DD groups than in the CC group. We found that the downregulation of the pyruvate dehydrogenase complex (PDC) gene and the upregulation phosphoenolpyruvate carboxykinase (PPC) gene were involved in energy metabolism processes in LL or DD. In addition, we also found that the upregulation of the expression level of the genes Gαq, pyruvate kinase (PK), NADH peroxidase (NADH) and ATPase is involved in phototransduction and energy metabolism. These results may shed some light on the molecular mechanism underlying the effect of photoperiod in physiological activity of E. sinensis.