Therapeutic effect of localized vibration on alveolar bone of osteoporotic rats

• Published in: PloS one 2019-01, Vol.14 (1), p.e0211004-e0211004
• Main Authors: Alikhani, Mani, Alikhani, Mona, Alansari, Sarah, Almansour, Abdullah, Hamidaddin, Mohammad A, Khoo, Edmund, Lopez, Jose A, Nervina, Jeanne M, Nho, Joo Y, Oliveira, Serafim M, Sangsuwon, Chinapa, Teixeira, Cristina C
• Format: Article
• Language: English
• Subjects: Alveolar bone; Alveolar process; Animals; Biocompatibility; Biology and Life Sciences; Bone density; Bone growth; Bone healing; Bones; Care and treatment; Cbfa-1 protein; Collagen; Computed tomography; Dental materials; Dentistry; Disease; DNA binding proteins; Electrotherapeutics; Female; Femur; Femur Head - diagnostic imaging; Femur Head - metabolism; Fluorescence; FOXO1 protein; Gene expression; Health aspects; Histology; Lumbar Vertebrae - diagnostic imaging; Lumbar Vertebrae - metabolism; Maxilla - diagnostic imaging; Maxilla - metabolism; Mechanical properties; Mechanical tests; Medicine and Health Sciences; Metabolism; Microscopy; Nutrient deficiency; Oophorectomy; Orthodontics; Osteogenesis; Osteoporosis; Osteoporosis - diagnostic imaging; Osteoporosis - metabolism; Osteoporosis - therapy; Ovariectomy; Patient outcomes; Physical Sciences; Physiological aspects; Proteins; Rats; Rats, Sprague-Dawley; Research and Analysis Methods; Ribonucleic acid; RNA; SOST protein; Stem cells; Surgery; Teeth; TRANCE protein; Transcription factors; Vertebrae; Vibration; Vibration - therapeutic use; Vibration analysis; Vibration control; Wnt protein; Womens health; X-Ray Microtomography
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0211004

Vibration, in the form of high frequency acceleration (HFA), stimulates alveolar bone formation under physiologic conditions and during healing after dental extractions. It is not known if HFA has an anabolic effect on osteoporotic alveolar bone. Our objective is to determine if HFA has a regenerative effect on osteoporotic alveolar bone. Adult female Sprague-Dawley rats were divided into five groups: 1) Ovariectomized Group (OVX), 2) Sham-OVX Group that received surgery without ovariectomy, 3) OVX-HFA Group that was ovariectomized and treated daily with HFA, 4) OVX+Static Force Group that was ovariectomized and received the same force as HFA, but without vibration, and 5) Control Group that did not receive any treatment. All animals were fed a low mineral diet for 3 months. Osteoporosis was confirmed by micro-CT of the fifth lumbar vertebra and femoral head. HFA was applied to the maxillary first molar for 5 minutes/day for 28 and 56 days. Maxillae were collected for micro-CT, histology, fluorescent microscopy, protein and RNA analysis, and three-point bending mechanical testing. Micro-CT analysis revealed significant alveolar bone osteoporosis in the OVX group. Vibration restored the quality and quantity of alveolar bone to levels similar to the Sham-OVX group. Animals exposed to HFA demonstrated higher osteoblast activity and lower osteoclast activity. Osteogenic transcription factors (RUNX2, Foxo1, Osterix and Wnt signaling factors) were upregulated following vibration, while RANKL/RANK and Sclerostin were downregulated. HFA did not affect serum TRAcP-5b or CTx-1 levels. The osteogenic effect was highest at the point of HFA application and extended along the hemimaxillae this effect did not cross to the contra-lateral side. Local application of vibration generated gradients of increased anabolic metabolism and decreased catabolic metabolism in alveolar bone of osteoporotic rats. Our findings suggest that HFA could be a predictable treatment for diminished alveolar bone levels in osteoporosis patients.