Optimization of irradiation dose to Aedes aegypti and Ae. albopictus in a sterile insect technique program

• Published in: PloS one 2019-02, Vol.14 (2), p.e0212520-e0212520
• Main Authors: Bond, J Guillermo, Osorio, Adriana R, Avila, Nancy, Gómez-Simuta, Yeudiel, Marina, Carlos F, Fernández-Salas, Ildefonso, Liedo, Pablo, Dor, Ariane, Carvalho, Danilo O, Bourtzis, Kostas, Williams, Trevor
• Format: Article
• Language: English
• Subjects: Aedes - radiation effects; Aedes aegypti; Aedes albopictus; Analysis; Animals; Aquatic insects; Biology and Life Sciences; Climate change; Competitiveness; Disease transmission; Egg industry; Egg production; Eggs; Females; Fertility; Fertility - radiation effects; Follicles; Infertility; Insecta; Insects; Ionizing radiation; Irradiation; Laboratories; Male; Males; Medicine and Health Sciences; Methods; Mexico; Mosquito Control - methods; Mosquito Vectors; Mosquitoes; Nuclear energy; Optimization; People and places; Pest control; Public health; Radiation; Radiation (Physics); Radiation Dosage; Radiation, Ionizing; Radiotherapy Dosage; Sexual Behavior, Animal - radiation effects; Species; Sterilization, Reproductive - methods; Sterilized organisms; Survival; Tropical diseases; Vectors; Zika virus
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0212520

The sterile insect technique (SIT) may offer a means to control the transmission of mosquito borne diseases. SIT involves the release of male insects that have been sterilized by exposure to ionizing radiation. We determined the effects of different doses of radiation on the survival and reproductive capacity of local strains of Aedes aegypti and Ae. albopictus in southern Mexico. The survival of irradiated pupae was invariably greater than 90% and did not differ significantly in either sex for either species. Irradiation had no significant adverse effects on the flight ability (capacity to fly out of a test device) of male mosquitoes, which consistently exceeded 91% in Ae. aegypti and 96% in Ae. albopictus. The average number of eggs laid per female was significantly reduced in Ae. aegypti at doses of 15 and 30 Gy and no eggs were laid by females that had been exposed to 50 Gy. Similarly, in Ae. albopictus, egg production was reduced at doses of 15 and 25 Gy and was eliminated at 35 Gy. In Ae. aegypti, fertility in males was eliminated at 70 Gy and was eliminated at 30 Gy in females, whereas in Ae. albopictus, the fertility of males that mated with untreated females was almost zero (0.1%) in the 50 Gy treatment and female fertility was eliminated at 35 Gy. Irradiation treatments resulted in reduced ovary length and fewer follicles in both species. The adult median survival time of both species was reduced by irradiation in a dose-dependent manner. However, sterilizing doses of 35 Gy and 50 Gy resulted in little reduction in survival times of males of Ae. albopictus and Ae. aegypti, respectively, indicating that these doses should be suitable for future evaluations of SIT-based control of these species. The results of the present study will be applied to studies of male sexual competitiveness and to stepwise evaluations of the sterile insect technique for population suppression of these vectors in Mexico.