Temporal dynamics of bacterial and fungal communities during the infection of Brassica rapa roots by the protist Plasmodiophora brassicae

• Published in: PloS one 2019-02, Vol.14 (2), p.e0204195-e0204195
• Main Authors: Lebreton, Lionel, Guillerm-Erckelboudt, Anne-Yvonne, Gazengel, Kévin, Linglin, Juliette, Ourry, Morgane, Glory, Pascal, Sarniguet, Alain, Daval, Stéphanie, Manzanares-Dauleux, Maria J, Mougel, Christophe
• Format: Article
• Language: English
• Subjects: Acids; Bacteria; Bacteria - genetics; Bacterial genetics; Biodiversity; Biology and Life Sciences; Brain; Brassica; Brassica oleracea; Brassica rapa - microbiology; Brassica rapa - parasitology; Chinese cabbage; Chytridiomycota; Clubroot; Cortex; Deoxyribonucleic acid; Disease Progression; Diseases and pests; DNA; DNA sequencing; Ecology and Environmental Sciences; Fungi; Fungi - genetics; Gall; Gene sequencing; Infection; Infections; Inoculation; Life Sciences; Metabolites; Microbial activity; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Nutrition; Parasites; Parasitic diseases; Physiological aspects; Physiology; Plant Diseases - microbiology; Plant Diseases - parasitology; Plant growth; Plant roots; Plant Roots - microbiology; Plasmodiophora brassicae; Plasmodiophorida; Rhizosphere; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 18S - genetics; Roots; Sampling; Signs and symptoms; Soil Microbiology; Soil structure; Symbiosis; Time Factors; Vegetal Biology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0204195

The temporal dynamics of rhizosphere and root microbiota composition was compared between healthy and infected Chinese cabbage plants by the pathogen Plasmodiophora brassicae. When inoculated with P. brassicae, disease was measured at five sampling dates from early root hair infection to late gall development. The first symptoms of clubroot disease appeared 14 days after inoculation (DAI) and increased drastically between 14 and 35 DAI. The structure of microbial communities associated to rhizosphere soil and root from healthy and inoculated plants was characterized through high-throughput DNA sequencing of bacterial (16S) and fungal (18S) molecular markers and compared at each sampling date. In healthy plants, Proteobacteria and Bacteroidetes bacterial phyla dominated the rhizosphere and root microbiota of Chinese cabbage. Rhizosphere bacterial communities contained higher abundances of Actinobacteria and Firmicutes compared to the roots. Moreover, a drastic shift of fungal communities of healthy plants occurred between the two last sampling dates, especially in plant roots, where most of Ascomycota fungi dominated until they were replaced by a fungus assigned to the Chytridiomycota phylum. Parasitic invasion by P. brassicae disrupted the rhizosphere and root-associated community assembly at a late step during the root secondary cortical infection stage of clubroot disease. At this stage, Flavisolibacter and Streptomyces in the rhizosphere, and Bacillus in the roots, were drastically less abundant upon parasite invasion. Rhizosphere of plants colonized by P. brassicae was significantly more invaded by the Chytridiomycota fungus, which could reflect a mutualistic relationship in this compartment between these two microorganisms.